Common nuclear matrix proteins in rat tissues

Electrophoresis
T KorosecG Sauermann

Abstract

Nuclear matrix proteins have been defined as insoluble residual proteins resulting from treatment of isolated nuclei with nucleases, detergents and high ionic strength buffers. They are considered as in part representing the proteins constituting the three-dimensional framework of the interphase nucleus. Though cell-specific nuclear matrix proteins have been differentiated from ubiquitously occurring (common) nuclear matrix proteins, the number and types of common nuclear matrix proteins have not yet been unequivocally established. In the present study nuclear matrix proteins were prepared from isolated nuclei of rat kidney, liver, lung, spleen and testes. The matrix proteins were separated by two-dimensional (2-D) electrophoresis and silver stained. Then the spot patterns were compared by computer-assisted image analysis. Composite images were derived for nuclear matrix proteins of individual tissues. Finding between 396-483 spots per tissue, a total of 964 individual spots were registered. Of these, 102 were common nuclear matrix proteins, as appearing in each of the tissue-characteristic images. The apparent molecular mass and pI data may serve for further identification of these nuclear proteins.

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Citations

Nov 27, 1998·Journal of Cellular Biochemistry·C GernerG Sauermann
Feb 2, 2000·Journal of Cellular Biochemistry·M MeissnerG Sauermann
May 14, 2004·Biology of Reproduction·Reiko MyojinYasuyuki Kurihara
Jan 7, 2000·Biochemical and Biophysical Research Communications·K HolzmannG Sauermann
Apr 6, 2007·Cell Biochemistry and Function·Nevena GrdovićGoran Poznanović
Mar 14, 1998·Experimental Cell Research·C GernerG Sauermann
Nov 14, 1998·Biochemical and Biophysical Research Communications·K HolzmannG Sauermann

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