Common structural basis for constitutive activity of the ghrelin receptor family.

The Journal of Biological Chemistry
Birgitte HolstThue W Schwartz

Abstract

Three members of the ghrelin receptor family were characterized in parallel: the ghrelin receptor, the neurotensin receptor 2 and the orphan receptor GPR39. In transiently transfected COS-7 and human embryonic kidney 293 cells, all three receptors displayed a high degree of ligand-independent signaling activity. The structurally homologous motilin receptor served as a constitutively silent control; upon agonist stimulation, however, it signaled with a similar efficacy to the three related receptors. The constitutive activity of the ghrelin receptor and of neurotensin receptor 2 through the G(q), phospholipase C pathway was approximately 50% of their maximal capacity as determined through inositol phosphate accumulation. These two receptors also showed very high constitutive activity in activation of cAMP response element-driven transcription. GPR39 displayed a clear but lower degree of constitutive activity through the inositol phosphate and cAMP response element pathways. In contrast, GPR39 signaled with the highest constitutive activity in respect of activation of serum response element-dependent transcription, in part, possibly, through G(12/13) and Rho kinase. Antibody feeding experiments demonstrated that the epitope-tagge...Continue Reading

References

Aug 1, 1994·Current Opinion in Biotechnology·T W Schwartz
Apr 21, 1999·Current Opinion in Cell Biology·L M LuttrellR J Lefkowitz
Oct 27, 1999·Proceedings of the National Academy of Sciences of the United States of America·C E EllingT W Schwartz
Apr 15, 2000·Nature·M W SchwartzD G Baskin
Nov 1, 2000·Nature·M TschöpM L Heiman
Apr 27, 2001·Endocrine·R G SmithA Howard
Dec 1, 2001·The Journal of Biological Chemistry·Stéphanie Miserey-LenkeiEric Clauser
Jan 5, 2002·Journal of Virology·Yvonne K GruijthuijsenCornelis Vink
Mar 30, 2002·The Journal of Biological Chemistry·Evgeni G PonimaskinTatyana A Voyno-Yasenetskaya
Aug 9, 2002·Nature·Michael W Schwartz, Gregory J Morton
Sep 10, 2002·Trends in Endocrinology and Metabolism : TEM·Charles ParnotEric Clauser
Oct 17, 2002·Naunyn-Schmiedeberg's Archives of Pharmacology·Roland Seifert, Katharina Wenzel-Seifert
Feb 8, 2003·Annual Review of Biophysics and Biomolecular Structure·Slawomir FilipekRonald Stenkamp
Feb 15, 2003·Endocrinology·Kichoon ChoiShin-Ichi Sasaki
Mar 12, 2003·Biochemistry·Tara MirzadeganKrzysztof Palczewski
Jun 13, 2003·Life Sciences·Paul R DobnerJim Fadel
Jul 10, 2003·Annals of the New York Academy of Sciences·Birgitte Holst, Thue W Schwartz
Jul 11, 2003·The EMBO Journal·Claire SunyachRoger Morris
Aug 9, 2003·Molecular Endocrinology·Birgitte HolstThue W Schwartz
Sep 30, 2003·The Journal of Biological Chemistry·Angelika KrebsGebhard F X Schertler
Mar 6, 2004·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Sandra H ChangZhengui Xia

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Citations

Jul 2, 2011·Psychopharmacology·F Scott HallGeorge R Uhl
Sep 4, 2009·Inflammation·Kevin B S ChowHelen Wise
Mar 23, 2011·Endocrine·Jiang-Bo LiAkio Inui
Mar 31, 2011·Journal of Medicinal Chemistry·Jürgen EinsiedelPeter Gmeiner
Aug 28, 2012·Journal of Medicinal Chemistry·Sylvia ElsAnnette G Beck-Sickinger
May 11, 2012·Proceedings of the National Academy of Sciences of the United States of America·Sophie MaryJean-Louis Banères
Jun 23, 2012·Proceedings of the National Academy of Sciences of the United States of America·Shingo UenoTakafumi Sakai
Nov 19, 2009·The Journal of Biological Chemistry·Birgitte HolstThue W Schwartz
Mar 16, 2011·The Journal of Biological Chemistry·Bjørn SivertsenBirgitte Holst
Aug 10, 2012·The Journal of Biological Chemistry·Tau Benned-JensenMette M Rosenkilde
Feb 25, 2010·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Gunnar KleinauGerd Krause
Jul 2, 2009·Clinical and Experimental Pharmacology & Physiology·Inge SeimAdrian C Herington
Dec 24, 2004·Molecular Interventions·Kenneth A JacobsonZhan-Guo Gao
Jan 13, 2006·Annual Review of Pharmacology and Toxicology·Thue W SchwartzChristian E Elling
Oct 13, 2006·Annual Review of Pharmacology and Toxicology·Martine J SmitRob Leurs
May 31, 2006·Hormone Research·Jean-Pierre ChanoineVicente Barrios
Mar 3, 2006·The Journal of Clinical Investigation·Jacques PantelSerge Amselem
Dec 3, 2005·The Journal of Clinical Investigation·Kevin L Grove, Michael A Cowley
Mar 3, 2006·The Journal of Clinical Investigation·Birgitte Holst, Thue W Schwartz
Mar 22, 2013·Therapeutic Advances in Endocrinology and Metabolism·Jacqueline A Bayliss, Zane B Andrews
Aug 7, 2012·Endocrinology·Kristy M HeppnerMatthias H Tschöp
Mar 6, 2009·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Limor BesserMichal Hershfinkel
Feb 19, 2010·Folia Histochemica Et Cytobiologica·Andrzej ŁukaszykJerzy Sowiński
Apr 24, 2012·Journal of Neurogastroenterology and Motility·Andreas Stengel, Yvette Taché
Mar 22, 2014·International Journal of Molecular Sciences·Yue YinWeizhen Zhang
Jul 25, 2007·Journal of Endocrinological Investigation·M J IglesiasF Lago
Dec 20, 2013·Journal of Molecular Endocrinology·Hiroyuki KaiyaMikiya Miyazato
May 23, 2013·Pharmacology & Therapeutics·Stefan AmistenShanta J Persaud
Aug 28, 2010·International Journal of Peptides·Johannes D Veldhuis, Cyril Y Bowers
Sep 3, 2010·Cellular and Molecular Life Sciences : CMLS·Petra Popovics, Alan J Stewart
Jul 1, 2008·FEBS Letters·Laura StorjohannThue W Schwartz
Jan 21, 2015·Progress in Neuro-psychopharmacology & Biological Psychiatry·Luana M ManossoAna Lúcia S Rodrigues
Apr 7, 2015·Physiology & Behavior·J R StevensonA E Bohidar
Oct 24, 2012·Behavioural Brain Research·Katarzyna MłyniecGabriel Nowak
May 28, 2011·Molecular and Cellular Endocrinology·Inge SeimLisa Chopin
Mar 27, 2010·Peptides·Kristina SjölundNils Wierup
Sep 24, 2015·Nature Reviews. Gastroenterology & Hepatology·Gareth J Sanger, John B Furness

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