Common Structural Elements in the Chromophore Binding Pocket of the Pfr State of Bathy Phytochromes

Photochemistry and Photobiology
Francisco Velazquez EscobarP Hildebrandt

Abstract

Phytochromes are bimodal photoreceptors which, upon light absorption by the tetrapyrrole chromophore, can be converted between a red-absorbing state (Pr) and far-red-absorbing state (Pfr). In bacterial phytochromes, either Pr or Pfr are the thermally stable states, thereby constituting the classes of prototypical and bathy phytochromes, respectively. In this work, we have employed vibrational spectroscopies to elucidate the origin of the thermal stability of the Pfr states in bathy phytochromes. Here, we present the first detailed spectroscopic analysis of RpBphP6 (Rhodopseudomas palustris), which together with results obtained for Agp2 (Agrobacterium tumefaciens) and PaBphP (Pseudomonas aeruginosa) allows identifying common structural properties of the Pfr state of bathy phytochromes, which are (1) a homogenous chromophore structure, (2) the protonated ring C propionic side chain of the chromophore and (3) a retarded H/D exchange at the ring D nitrogen. These properties are related to the unique strength of the hydrogen bonding interactions between the ring D N-H group with the side chain of the conserved Asp194 (PaBphP numbering). As revealed by a comparative analysis of homology models and available crystal structures of Pfr...Continue Reading

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Citations

Feb 15, 2019·Biological Chemistry·Birthe StüvenAndreas Möglich
Nov 23, 2018·Nature Communications·Andrea SchmidtPatrick Scheerer
Feb 26, 2021·Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy·Faez Iqbal KhanFeng Juan
Mar 14, 2019·Biological Chemistry·Birthe StüvenAndreas Möglich
May 18, 2021·Frontiers in Plant Science·Tilman LamparterNorbert Krauß
Feb 20, 2020·Biochemistry·Anastasia KraskovPeter Hildebrandt
Jul 28, 2019·Biochemistry·Maria Fernandez LopezPeter Hildebrandt
Nov 30, 2021·The Journal of Physical Chemistry. B·Anastasia KraskovPeter Hildebrandt

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