Commonly used trafficking blocks disrupt ARF1 activation and the localization and function of specific Golgi proteins

Molecular Biology of the Cell
Catherine E GilbertCarolyn E Machamer

Abstract

Cold temperature blocks used to synchronize protein trafficking inhibit GBF1 function, leading to a decrease in ARF1-GTP levels and mislocalization of the ARF1 effector golgin-160. Several other, but not all, Golgi proteins including ARL1 also mislocalize. ARF1 activity and golgin-160 localization require more than 30 min to recover from these blocks.

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Citations

Jul 31, 2020·Journal of Integrative Plant Biology·Lu LiuHao Yu
Aug 22, 2020·International Braz J Urol : Official Journal of the Brazilian Society of Urology·Haiming LeiBo Tan
Jun 11, 2020·American Journal of Physiology. Cell Physiology·Skylar FisherElizabeth Sztul
May 16, 2019·Molecular Biology of the Cell·Elizabeth SztulRichard A Kahn
Sep 3, 2018·Histochemistry and Cell Biology·Jaakko Saraste, Michaël Marie
Sep 10, 2020·Journal of Bacteriology·Alfa Herrera, Karla J F Satchell
Jan 17, 2021·Applied and Environmental Microbiology·Chenyu WangHansong Dong

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Datasets Mentioned

BETA
GM130

Methods Mentioned

BETA
GTPase
nucleotide exchange
pull-down
fluorescence microscopy
transfection
GTPases
fluorescence recovery after photobleaching
glycosylation
in
Assay

Software Mentioned

GraphPad Prism
Quantity One
CUTE
RUSH
iVision
GraphPad
Robust Regression and Outlier removal ( ROUT )
Image Studio
LARIAT
Prism

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