Communication between subunits within an archaeal clamp-loader complex.

The EMBO Journal
Anja SeybertDale B Wigley

Abstract

We have investigated the communication between subunits in replication factor C (RFC) from Archaeoglobus fulgidus. Mutation of the proposed arginine finger in the small subunits results in a complex that can still bind ATP but has impaired clamp-loading activity, a process that normally only requires binding of nucleotide. The small subunit alone forms a hexameric ring that is six-fold symmetric in the absence of ATP. However, this symmetry is broken when the nucleotide is bound to the complex. A conformational change associated with nucleotide binding may relate to the opening of PCNA rings by RFC during the loading reaction. The structures also reveal the importance of the N-terminal helix of each subunit at the ATP-binding site. Analysis of mutant protein complexes containing subunits lacking this N-terminal helix reveals key distinct regulatory roles during clamp loading that are different for the large and small subunits in the RFC complex.

References

Sep 1, 1995·Molecular and Cellular Biology·G CullmannB Stillman
Sep 26, 1997·Nature Structural Biology·M R AhmadianA Wittinghofer
Oct 3, 1998·Acta Crystallographica. Section D, Biological Crystallography·A T BrüngerG L Warren
Feb 2, 1999·The EMBO Journal·J TurnerM O'Donnell
Mar 25, 1999·Acta Crystallographica. Section D, Biological Crystallography·T C Terwilliger, J Berendzen
Dec 20, 2000·Proceedings of the National Academy of Sciences of the United States of America·Y ShiomiT Tsurimoto
Jul 27, 2001·Journal of Structural Biology·K MayanagiK Morikawa
Jul 28, 2001·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·T Ogura, A J Wilkinson
Apr 18, 2002·Current Opinion in Structural Biology·David JeruzalmiJohn Kuriyan
Feb 13, 2003·The Journal of Biological Chemistry·Aaron Johnson, Mike O'Donnell
Jun 18, 2004·Nature·Gregory D BowmanJohn Kuriyan
Jun 23, 2004·Nature Structural & Molecular Biology·Tomoko MiyataKosuke Morikawa
Sep 1, 1994·Acta Crystallographica. Section D, Biological Crystallography·UNKNOWN Collaborative Computational Project, Number 4
May 1, 1997·Acta Crystallographica. Section D, Biological Crystallography·G N MurshudovE J Dodson
Sep 20, 2005·Proceedings of the National Academy of Sciences of the United States of America·Tomoko MiyataKosuke Morikawa
Feb 16, 2006·Proceedings of the National Academy of Sciences of the United States of America·Zhihao ZhuangStephen J Benkovic
Jan 1, 1997·Methods in Enzymology·Zbyszek Otwinowski, Wladek Minor

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Citations

Sep 7, 2006·Nature Reviews. Molecular Cell Biology·Chiara Indiani, Mike O'Donnell
Oct 14, 2008·Nature Structural & Molecular Biology·Xiaodong Zhang, Dale B Wigley
Jun 21, 2013·Nucleic Acids Research·Lin WangStephen J Benkovic
Apr 3, 2013·Cold Spring Harbor Perspectives in Biology·Mark HedglinStephen J Benkovic
Dec 24, 2011·Science·Brian A KelchJohn Kuriyan
Dec 13, 2006·Microbiology and Molecular Biology Reviews : MMBR·Elizabeth R Barry, Stephen D Bell
Jun 24, 2008·Critical Reviews in Biochemistry and Molecular Biology·Karl E Duderstadt, James M Berger
Jul 7, 2009·Biochimica Et Biophysica Acta·Zhihao Zhuang, Yongxing Ai
Mar 17, 2009·Journal of Molecular Biology·Siying ChenManju M Hingorani
Jul 24, 2008·Molecular Microbiology·Nathan D Thomsen, James M Berger
Dec 27, 2011·Journal of Molecular Biology·Miho SakatoManju M Hingorani
Apr 9, 2013·Cell·Ernesto Arias-PalomoJames M Berger
Oct 6, 2016·Archaea : an International Microbiological Journal·Justin M Miller, Eric J Enemark
Jul 29, 2018·Archives of Virology·Laurane PallandreLaurent Bigarré
Apr 22, 2014·Journal of Bacteriology·Xuanxuan XingLi Huang

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