Community analysis of arbuscular mycorrhizal fungi associated with Ammophila arenaria in Dutch coastal sand dunes

Molecular Ecology
George A KowalchukJohannes A van Veen

Abstract

A polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE) approach for the detection and characterization of arbuscular mycorrhizal fungi (AMF) 18S ribosomal DNA (rDNA) was developed and applied to the study of AMF communities associated with the main sand-stabilizing plant species of the Dutch sand dunes, marram grass (Ammophila arenaria, L.). DNA was extracted directly from plant roots, soil or isolated AMF spores, and prominent bands resulting from AMF-specific DGGE profiles were excised for sequence analysis. This strategy provided a robust means of detecting and identifying AMF-like species without the use of trap plant cultivation methods. A number of Glomus-like and Scutellospora-like sequences was detected, including a putatively novel Glomus species, and differences were observed in the dominant AMF-like populations detected in healthy vs. degenerating stands of A. arenaria and in bulk sand dune soil. It has previously been suggested that plant pathogens, such as fungi and nematodes, may contribute to the decline of A. arenaria. Although no causal relationship can be drawn between the observed differences in the dominantly detected AMF-like populations and the vitality of plant growth, these result...Continue Reading

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Citations

Oct 18, 2006·Mycorrhiza·Susana Rodríguez-Echeverría, Helena Freitas
Aug 6, 2005·Mycological Research·Francisco Adriano De SouzaGeorge A Kowalchuk
Jul 11, 2013·World Journal of Microbiology & Biotechnology·N WatanarojanapornN Teaumroong
Sep 4, 2008·Journal of Environmental Sciences (China)·Ruyi YangXin Chen
Feb 18, 2009·Environmental Microbiology·Petra WildeHermann Bothe
Jan 31, 2006·The New Phytologist·Victoria A Borowicz
Sep 11, 2012·Journal of Basic Microbiology·Young-Hyun YouJong-Guk Kim
Sep 1, 2010·Evolutionary Applications·Erik Verbruggen, E Toby Kiers
Jun 15, 2011·Molecular Ecology·Saad El Din HassanMohamed Hijri
Dec 14, 2004·Journal of Microbiological Methods·E YergeauM St-Arnaud
Jan 18, 2006·Current Opinion in Biotechnology·Jim I ProsserKen Killham
Jan 6, 2009·FEMS Microbiology Ecology·Yongjun LiuHuyuan Feng
Oct 27, 2015·Plant Biology·M T WellingK Benkendorff
Apr 29, 2009·Mycologia·Janusz BłaszkowskiTímea Balázs
Jan 9, 2019·PloS One·Paulo Prates JúniorMaria Catarina Megumi Kasuya
Aug 2, 2017·Journal of the Science of Food and Agriculture·Masao HigoKatsunori Isobe

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