Community analysis of the bacterial assemblages in the winter cover and pelagic layers of a high mountain lake by in situ hybridization.

Applied and Environmental Microbiology
A AlfreiderR Psenner

Abstract

The bacterial community structure in the winter cover and pelagic zone of a high mountain lake was analyzed by in situ hybridization with fluorescently labeled rRNA-targeted oligonucleotide probes. Cells fixed on membrane filters were hybridized with a probe specific for the domain Bacteria as well as with probes for the alpha, beta, and gamma subclasses of the class Proteobacteria and the Cytophaga-Flavobacterium group. The fraction of bacteria detectable after hybridization with the bacterial probe EUB ranged from 40 to 81% of 4(prm1),6-diamidino-2-phenylindole (DAPI) counts. The bacterial assemblage varied considerably between and within different habitats (snow, slush, and lake water) but was in most cases dominated by members of the beta subclass (6.5 to 116% of bacteria detectable with probe EUB). The sum of bacteria hybridizing with group-specific probes was usually lower than the fraction detectable with probe EUB. Image analysis was used to characterize morphology and the size-specific biomass distribution of bacterial assemblages, which clearly separated the three habitats. Although the measured secondary production parameters and the fraction of 2-(p-iodophenyl)-3-(p-nitrophenyl)-5-phenyltetrazolium chloride-reducing...Continue Reading

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Citations

Oct 31, 2002·Applied and Environmental Microbiology·J F HeidelbergR R Colwell
Jan 21, 2006·Environmental Microbiology·Holger DaimsMichael Wagner
Nov 17, 2006·FEMS Microbiology Letters·Yongqin LiuSijun Huang
Mar 3, 2007·FEMS Microbiology Ecology·Pierre AmatoChristophe Ferrari
Dec 1, 2002·FEMS Microbiology Ecology·Y BettarelT Sime-Ngando
Feb 26, 2010·FEMS Microbiology Ecology·Thomas R NeuJohn R Lawrence
Feb 1, 2012·Environmental Microbiology Reports·Tomàs Llorens-MarèsEmilio O Casamayor
Apr 5, 2013·The ISME Journal·Katherina HellBirgit Sattler
Jul 6, 2000·Applied and Environmental Microbiology·I H BrümmerI Wagner-Döbler
Jun 1, 2000·Applied and Environmental Microbiology·A S Whiteley, M J Bailey
Mar 19, 2005·Extremophiles : Life Under Extreme Conditions·Hisako HirayamaKoki Horikoshi
May 1, 2007·Applied and Environmental Microbiology·Jill A Mikucki, John C Priscu
Jun 10, 2010·FEMS Microbiology Ecology·Paul HörtnaglRuben Sommaruga
Jan 23, 2009·Extremophiles : Life Under Extreme Conditions·Yongqin LiuXiaobo Liu
May 8, 2014·Canadian Journal of Microbiology·Lei ZhangKeqiang Shao
Oct 15, 2013·Environmental Microbiology·Caroline L MonteilCindy E Morris
Feb 18, 2003·Environmental Microbiology·Graeme W NicolJames I Prosser
Aug 7, 2003·Applied and Environmental Microbiology·Fumito MaruyamaMasao Nasu
Oct 31, 1998·Applied and Environmental Microbiology·J PernthalerR Amann
Jan 4, 2007·FEMS Microbiology Ecology·Tamar Barkay, Alexandre J Poulain
Sep 9, 2005·Microbiology and Molecular Biology Reviews : MMBR·Jakob Pernthaler, Rudolf Amann
Mar 3, 2007·Applied Microbiology and Biotechnology·Holger Daims, Michael Wagner

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