Community-level genetic profiles of actinomycetales in long-term biowaste-amended soils

Archives of Microbiology
Sonia Mokni-TliliHelmi Hamdi

Abstract

Actinomycetales is an order of actinobacteria that have an important role in the decomposition of organic matter. Their abundance and distribution can reflect a good level of soil fertility as well as biological activity. In this research study, actinomycetal diversity in soil was investigated under various field treatments with biowastes. Initially, unvegetated agricultural soil plots of 4 m2 had been annually amended with increasing rates of municipal solid waste compost (MSWC at 40, 80 and 120 t ha-1 year-1) and farmyard manure (FM at 40 and 120 t ha-1 year-1) for eight consecutive years. Control consisted of unamended soil and all treatments were distributed in four randomized complete blocks. At the end of the experimental period, total DNA was extracted from fresh topsoil samples (0-20 cm) then nested PCR-DGGE sequencing method was applied to assess the long-term effect of treatments on the diversity of actinomycetes. Analytical outcomes revealed the presence of ten actinomycetal families with Streptomycetaceae, Pseudonocardiaceae and Nocardioidaceae being the most dominant regardless to changes in experimental conditions. Besides, the long-term accumulation of both biowastes in soil affected the diversity of actinomyceta...Continue Reading

References

Jun 29, 1999·Current Opinion in Microbiology·G Muyzer
Jan 16, 2007·Applied and Environmental Microbiology·Nancy N PerreaultLyle G Whyte
Dec 1, 2002·FEMS Microbiology Ecology·Paolo MonciardiniStefano Donadio
Dec 22, 2009·Waste Management & Research : the Journal of the International Solid Wastes and Public Cleansing Association, ISWA·Sonia Mokni-TliliMohamed Nejib Marzouki
Jan 15, 2013·Journal of Microbiological Methods·Julia W NeilsonRaina M Maier
Jun 26, 2017·Ecotoxicology and Environmental Safety·Amjad AliZengqiang Zhang

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Datasets Mentioned

BETA
JF975567
JF975590
FJ662866
97
HQ231890
JF975568
JF975581
GMKU
367

Methods Mentioned

BETA
electrophoresis
PCR
soil treatments

Software Mentioned

BLAST
Image J
XLSTAT
Clustal X

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