Community Sewage Sensors towards Evaluation of Drug Use Trends: Detection of Cocaine in Wastewater with DNA-Directed Immobilization Aptamer Sensors

Scientific Reports
Zhugen YangBarbara Kasprzyk-Hordern

Abstract

Illicit drug use has a global concern and effective monitoring and interventions are highly required to combat drug abuse. Wastewater-based epidemiology (WBE) is an innovative and cost-effective approach to evaluate community-wide drug use trends, compared to traditional population surveys. Here we report for the first time, a novel quantitative community sewage sensor (namely DNA-directed immobilization of aptamer sensors, DDIAS) for rapid and cost-effective estimation of cocaine use trends via WBE. Thiolated single-stranded DNA (ssDNA) probe was hybridized with aptamer ssDNA in solution, followed by co-immobilization with 6-mercapto-hexane onto the gold electrodes to control the surface density to effectively bind with cocaine. DDIAS was optimized to detect cocaine at as low as 10 nM with a dynamic range from 10 nM to 5 μM, which were further employed for the quantification of cocaine in wastewater samples collected from a wastewater treatment plant in seven consecutive days. The concentration pattern of the sampling week is comparable with that from mass spectrometry. Our results demonstrate that the developed DDIAS can be used as community sewage sensors for rapid and cost-effective evaluation of drug use trends, and potent...Continue Reading

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Citations

Oct 5, 2018·Drug Testing and Analysis·Nahla A AbdelshafiRudolf J Schneider
Mar 8, 2020·Biosensors·Roberta D'AurelioIbtisam E Tothill
Sep 3, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Dorian MigońDariusz Suchy
Aug 18, 2017·Analytical Chemistry·Zhugen YangJonathan M Cooper

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Methods Mentioned

BETA
biosensor
biosensing
aptasensor
aptasensors
biosensors
chips

Software Mentioned

TargetLynx
MassLynx

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