Compact high-resolution endomicroscopy based on fiber bundles and image stitching

Optics Letters
Yina ChangShih Chi Chen

Abstract

In this Letter, we report a compact endomicroscope (ϕ=2.8  mm) based on a fiber bundle and a two-axis piezoelectric tube scanner, achieving a resolution of ∼1  μm and an imaging speed of 30-120 fps. Compared with distal end scanning systems, typical fiber-bundle-based endomicroscopes achieve a more compact envelope (ϕ∼1.5  mm) at the expense of compromised imaging quality. The resolution of fiber-bundle-based systems is largely limited by the diameter of the constituent fibers (ϕ∼5.0  μm), where each fiber serves as a single pixel, i.e., a sampling point, in the imaging system. To retrieve the lost information, we integrate a piezo tube scanner at the tip of the fiber bundle. Next, we rapidly scan the fiber tip over a range of ±2.5  μm and combine the signals obtained at different inter-fiber locations. Direct alignment and feature-based registration methods are applied to register the raw images. Imaging experiments are performed on a resolution target and biological samples to demonstrate the performance enhancement.

References

Oct 26, 2005·Gastrointestinal Endoscopy·Adrian L PolglasePeter M Delaney
Nov 22, 2005·Nature Methods·Benjamin A FlusbergMark J Schnitzer
Oct 6, 2006·IEEE Transactions on Bio-medical Engineering·Christian WinterThomas Wittenberg
Oct 7, 2008·Nature Methods·Benjamin A FlusbergMark J Schnitzer
Dec 10, 2007·Optics Express·Timothy J MuldoonRebecca Richards-Kortum
Aug 1, 2014·Optics Letters·Gyeong Woo CheonJin U Kang
Feb 13, 2015·Journal of Microelectromechanical Systems : a Joint IEEE and ASME Publication on Microstructures, Microactuators, Microsensors, and Microsystems·Shih-Chi ChenMartin L Culpepper

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Citations

Mar 21, 2019·Biomedical Optics Express·Hamin JeonTomasz S Tkaczyk

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