Compact soft x-ray transmission microscopy with sub-50 nm spatial resolution

Physics in Medicine and Biology
Kyong Woo KimKwon-Ha Yoon

Abstract

In this paper, the development of compact transmission soft x-ray microscopy (XM) with sub-50 nm spatial resolution for biomedical applications is described. The compact transmission soft x-ray microscope operates at lambda = 2.88 nm (430 eV) and is based on a tabletop regenerative x-ray source in combination with a tandem ellipsoidal condenser mirror for sample illumination, an objective micro zone plate and a thinned back-illuminated charge coupled device to record an x-ray image. The new, compact x-ray microscope system requires the fabrication of proper x-ray optical devices in order to obtain high-quality images. For an application-oriented microscope, the alignment procedure is fully automated via computer control through a graphic user interface. In imaging studies using our compact XM system, a gold mesh image was obtained with 45 nm resolution at x580 magnification and 1 min exposure. Images of a biological sample (Coscinodiscus oculoides) were recorded.

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Citations

Sep 1, 2006·Photomedicine and Laser Surgery
Sep 17, 2015·Microscopy and Microanalysis : the Official Journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada·Przemyslaw WachulakHenryk Fiedorowicz
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Jan 1, 2009·Journal of Physics. Conference Series·S F HorneW Holber
Nov 4, 2021·Cell Reports Methods·Valentina LoconteVenera Weinhardt

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