Compaction and multiple chain assembly of DNA with the cationic polymer poly(aluminum chloride) (PAC)

Langmuir : the ACS Journal of Surfaces and Colloids
Y MatsuzawaK Yoshikawa

Abstract

Assembly of DNA molecules by the addition of poly(aluminum chloride) (PAC) was studied. In the absence of PAC, electron microscopy indicated the formation of elongated coiled DNA molecules. In the presence of PAC, multiple doughnut-like structures, 8-15 nm thick, formed and fused together. When salt was added, the doughnut-like structures tended to be thinner and the morphology of the fused doughnuts became irregular. We obtained a view of a single DNA structure by fluorescent microscopy, which revealed that individual DNA molecules undergo a discrete transition from an elongated to compacted state with an increase in PAC concentration. Electron microscopic observation showed that a regular doughnut is the typical structure seen under low salt conditions. At high salt concentrations, the doughnut shape deformed, yielding results similar to those produced by the salt effect on DNA assembly at high DNA concentrations.

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Citations

Jul 23, 2005·The Journal of Chemical Physics·Y TakenakaT Kanbe
Aug 7, 2010·The Journal of Chemical Physics·Takuya Saito, Kenichi Yoshikawa
Feb 24, 2015·Journal of Biomedical Optics·Eileen S FloresMilind Rajadhyaksha
Oct 16, 2010·The Journal of Physical Chemistry. B·K FurusawaT Yamamoto
Jun 7, 2007·The Journal of Physical Chemistry. B·Antara DasguptaSouvik Maiti

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