Abstract
Assembly of DNA molecules by the addition of poly(aluminum chloride) (PAC) was studied. In the absence of PAC, electron microscopy indicated the formation of elongated coiled DNA molecules. In the presence of PAC, multiple doughnut-like structures, 8-15 nm thick, formed and fused together. When salt was added, the doughnut-like structures tended to be thinner and the morphology of the fused doughnuts became irregular. We obtained a view of a single DNA structure by fluorescent microscopy, which revealed that individual DNA molecules undergo a discrete transition from an elongated to compacted state with an increase in PAC concentration. Electron microscopic observation showed that a regular doughnut is the typical structure seen under low salt conditions. At high salt concentrations, the doughnut shape deformed, yielding results similar to those produced by the salt effect on DNA assembly at high DNA concentrations.
References
May 1, 1978·Quarterly Reviews of Biophysics·G S Manning
May 29, 1979·Biochemistry·R W Wilson, V A Bloomfield
Jan 1, 1990·Biopolymers·P G ArscottV A Bloomfield
Aug 5, 1973·Journal of Molecular Biology·D Lang
Aug 5, 1973·Journal of Molecular Biology·K E RichardsR Calendar
Aug 1, 1971·Proceedings of the National Academy of Sciences of the United States of America·L S Lerman
Dec 25, 1980·Journal of Molecular Biology·J Widom, R L Baldwin
Jun 3, 1994·Science·Z ReichA Minsky
Jun 30, 1993·Biochemical and Biophysical Research Communications·N V HudR Balhorn
Jul 1, 1996·Biophysical Journal·J PeltaF Livolant
Sep 2, 1996·Physical Review Letters·M Ueda, K Yoshikawa
Feb 24, 2000·Biopolymers·S HeV A Bloomfield
Dec 6, 2001·Proceedings of the National Academy of Sciences of the United States of America·N V Hud, K H Downing
Sep 12, 2002·Biophysical Chemistry·Naoko Makita, Kenichi Yoshikawa
Jul 23, 2004·The Journal of Chemical Physics·Toshio IwatakiSergey S Abramchuk
Citations
Jul 23, 2005·The Journal of Chemical Physics·Y TakenakaT Kanbe
Aug 7, 2010·The Journal of Chemical Physics·Takuya Saito, Kenichi Yoshikawa
Sep 5, 2014·Langmuir : the ACS Journal of Surfaces and Colloids·Xiaoxi HuangTewodros Asefa
Sep 30, 2010·The British Journal of Dermatology·A ScopeM Rajadhyaksha
Feb 24, 2015·Journal of Biomedical Optics·Eileen S FloresMilind Rajadhyaksha
Oct 16, 2010·The Journal of Physical Chemistry. B·K FurusawaT Yamamoto
Jun 7, 2007·The Journal of Physical Chemistry. B·Antara DasguptaSouvik Maiti