Comparative "-omics" in Mycoplasma pneumoniae Clinical Isolates Reveals Key Virulence Factors

PloS One
Maria Lluch-SenarLuis Serrano

Abstract

The human respiratory tract pathogen M. pneumoniae is one of the best characterized minimal bacterium. Until now, two main groups of clinical isolates of this bacterium have been described (types 1 and 2), differing in the sequence of the P1 adhesin gene. Here, we have sequenced the genomes of 23 clinical isolates of M. pneumoniae. Studying SNPs, non-synonymous mutations, indels and genome rearrangements of these 23 strains and 4 previously sequenced ones, has revealed new subclasses in the two main groups, some of them being associated with the country of isolation. Integrative analysis of in vitro gene essentiality and mutation rates enabled the identification of several putative virulence factors and antigenic proteins; revealing recombination machinery, glycerol metabolism and peroxide production as possible factors in the genetics and physiology of these pathogenic strains. Additionally, the transcriptomes and proteomes of two representative strains, one from each of the two main groups, have been characterized to evaluate the impact of mutations on RNA and proteins levels. This study has revealed that type 2 strains show higher expression levels of CARDS toxin, a protein recently shown to be one of the major factors of in...Continue Reading

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May 14, 2021·Journal of Clinical Microbiology·Jasna Rodman BerlotDarja Keše

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Datasets Mentioned

BETA
CP003913.1
CP002077.1
SRP061659
PXD002501

Methods Mentioned

BETA
nucleic acid amplification
PCR
chip
RNA-seq
RNAseq

Software Mentioned

FastQC
bwa mem
FoldX
gplots
fac
Abyss
Ensembl
Variant Effect Predictor
bcftools
Abyss package

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