Comparative analyses of the Conserved Oligomeric Golgi (COG) complex in vertebrates.

BMC Evolutionary Biology
Rita QuentalAntónio Amorim

Abstract

The Conserved Oligomeric Golgi (COG) complex is an eight-subunit assembly that localizes peripherally to Golgi membranes and is involved in retrograde vesicular trafficking. COG subunits are organized in two heterotrimeric groups, Cog2, -3, -4 and Cog5, -6, -7, linked by a dimeric group formed by Cog1 and Cog8. Dysfunction of COG complex in humans has been associated with new forms of Congenital Disorders of Glycosylation (CDG), therefore highlighting its essential role. In the present study, we intended to gain further insights into the evolution of COG subunits in vertebrates, using comparative analyses of all eight COG proteins. We used protein distances and dN/dS ratios as a measure of the rate of proteins evolution. The results showed that all COG subunits are evolving under strong purifying selection, although COG1 seems to evolve faster than the remaining proteins. In addition, we also tested the expression of COG genes in 20 human tissues, and demonstrate their ubiquitous nature. COG complex has a critical role in Golgi structure and function, which, in turn, is involved in protein sorting and glycosylation. The results of this study suggest that COG subunits are evolutionary constrained to maintain the interactions bet...Continue Reading

References

Oct 5, 1990·Journal of Molecular Biology·S F AltschulD J Lipman
Nov 3, 1994·Nature·J E Rothman
Feb 3, 1999·Proceedings of the National Academy of Sciences of the United States of America·J E ChattertonM Krieger
Apr 9, 2001·The Journal of Biological Chemistry·E S SuvorovaV V Lupashin
May 15, 2002·Molecular Biology of the Cell·Rachna J RamChris A Kaiser
Nov 26, 2002·Journal of Molecular Biology·Sarah A Teichmann
Mar 23, 2004·Nucleic Acids Research·Robert C Edgar
Mar 30, 2004·The Journal of Biological Chemistry·Eva Loh, Wanjin Hong
Apr 27, 2004·Nature Medicine·Xiaohua WuHudson H Freeze
Feb 25, 2005·The Journal of Cell Biology·Sergey N Zolov, Vladimir V Lupashin
Mar 8, 2005·Journal of Biochemistry·Toshihiko Oka, Monty Krieger
Jun 4, 2005·The Journal of Biological Chemistry·Pierre FotsoVladimir V Lupashin
Jun 28, 2005·Biochimica Et Biophysica Acta·Vladimir Lupashin, Elizabeth Sztul
Jul 16, 2005·The Journal of Biological Chemistry·Daniel UngarFrederick M Hughson
Jul 27, 2005·Proceedings of the National Academy of Sciences of the United States of America·Julian Mintseris, Zhiping Weng
Nov 16, 2005·Annual Review of Genetics·Eugene V Koonin
Jan 13, 2006·Trends in Cell Biology·Daniel UngarFrederick M Hughson
Mar 10, 2006·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Jose B Pereira-LealSarah A Teichmann
Mar 16, 2006·Proceedings of the National Academy of Sciences of the United States of America·François FoulquierGert Matthijs
Oct 6, 2006·Bioinformatics·S Blair HedgesSudhir Kumar
Mar 3, 2007·Human Molecular Genetics·Christian KranzHudson H Freeze
Mar 31, 2007·Molecular Genetics and Metabolism·Bobby G NgHudson H Freeze
May 8, 2007·Molecular Biology and Evolution·Ziheng Yang
Jun 15, 2007·The Journal of Biological Chemistry·Lorraine F CavanaughFrederick M Hughson
Nov 30, 2007·Nucleic Acids Research·David L WheelerEugene Yaschenko
Mar 21, 2008·Carbohydrate Research·Richard D Smith, Vladimir V Lupashin
Apr 10, 2008·Molecular Biology and Evolution·David Posada
Aug 20, 2008·PLoS Computational Biology·Teunis J P van Dam, Berend Snel
Nov 26, 2008·Biochimica Et Biophysica Acta·François Foulquier

❮ Previous
Next ❯

Methods Mentioned

BETA
glycosylation
PCR
electrophoresis
reverse transcription-PCR
GTPases

Software Mentioned

ClustalW
jModelTest
Ensembl genome browser
GBlocks
Phyml
TBLASTN
TimeTree
PHYLIP
Bioedit
MUSCLE

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.