Comparative analysis among the small RNA populations of source, sink and conductive tissues in two different plant-virus pathosystems

BMC Genomics
Mari Carmen HerranzVicente Pallás

Abstract

In plants, RNA silencing plays a fundamental role as defence mechanism against viruses. During last years deep-sequencing technology has allowed to analyze the sRNA profile of a large variety of virus-infected tissues. Nevertheless, the majority of these studies have been restricted to a unique tissue and no comparative analysis between phloem and source/sink tissues has been conducted. In the present work, we compared the sRNA populations of source, sink and conductive (phloem) tissues in two different plant virus pathosystems. We chose two cucurbit species infected with two viruses very different in genome organization and replication strategy; Melon necrotic spot virus (MNSV) and Prunus necrotic ringspot virus (PNRSV). Our findings showed, in both systems, an increase of the 21-nt total sRNAs together with a decrease of those with a size of 24-nt in all the infected tissues, except for the phloem where the ratio of 21/24-nt sRNA species remained constant. Comparing the vsRNAs, both PNRSV- and MNSV-infected plants share the same vsRNA size distribution in all the analyzed tissues. Similar accumulation levels of sense and antisense vsRNAs were observed in both systems except for roots that showed a prevalence of (+) vsRNAs in ...Continue Reading

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Citations

Nov 15, 2015·Plant Science : an International Journal of Experimental Plant Biology·Magdalena PawełkowiczZbigniew Przybecki
Apr 22, 2015·Journal of Proteomics·Marta Serra-SorianoVicente Pallás
Nov 17, 2012·PloS One·Ruimin GaoSek-Man Wong
Sep 13, 2016·Frontiers in Microbiology·Ahmed HadidiMarina Barba
Oct 4, 2016·Frontiers in Microbiology·Ida Bagus AndikaLiying Sun
Feb 20, 2019·BMC Plant Biology·Alejandro Sanz-CarbonellGustavo Gomez

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Datasets Mentioned

BETA
AJ306818

Methods Mentioned

BETA
dot-blot
PCR

Software Mentioned

ImageJ
BLAST
Primer Express®

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