Comparative analysis of alfalfa (Medicago sativa L.) leaf transcriptomes reveals genotype-specific salt tolerance mechanisms

BMC Plant Biology
Yunting LeiJianqiang Wu

Abstract

Soil salinity is an important factor affecting growth, development, and productivity of almost all land plants, including the forage crop alfalfa (Medicago sativa). However, little is known about how alfalfa responds and adapts to salt stress, particularly among different salt-tolerant cultivars. Among seven alfalfa cultivars, we found that Zhongmu-1 (ZM) is relatively salt-tolerant and Xingjiang Daye (XJ) is salt-sensitive. Compared to XJ, ZM showed slower growth under low-salt conditions, but exhibited stronger tolerance to salt stress. RNA-seq analysis revealed 2237 and 1125 differentially expressed genes (DEGs) between ZM and XJ in the presence and absence of salt stress, among which many genes are involved in stress-related pathways. After salt treatment, compared with the controls, the number of DEGs in XJ (19373) was about four times of that in ZM (4833). We also detected specific differential gene expression patterns: In response to salt stress, compared with XJ, ZM maintained relatively more stable expression levels of genes related to the ROS and Ca2+ pathways, phytohormone biosynthesis, and Na+/K+ transport. Notably, several salt resistance-associated genes always showed greater levels of expression in ZM than in XJ,...Continue Reading

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Citations

Oct 31, 2019·Journal of Experimental Botany·Shalan LiJianqiang Wu
Nov 30, 2019·Frontiers in Plant Science·Xiaoyan LvYuguang Wang
Jun 9, 2020·Frontiers in Plant Science·Miroslava HrbáčkováJozef Šamaj
May 15, 2021·Journal of the Science of Food and Agriculture·Guangxin CuiHuirong Duan

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Methods Mentioned

BETA
salt stress
RNA-seq
MDA
salt treatment

Software Mentioned

HMMER
DESeq2
Trinity
Btrim
BLAST
Bowite2

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