PMID: 11897799Mar 19, 2002Paper

Comparative analysis of an improved thioflavin-s stain, Gallyas silver stain, and immunohistochemistry for neurofibrillary tangle demonstration on the same sections

The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society
A SunG Bing

Abstract

An improved thioflavin-S stain, Gallyas silver stain, and two immunostainings were quantitatively compared for demonstration of neurofibrillary tangles (NFTs) on the same sections. Sections of hippocampal formation from seven cases of Alzheimer's disease (AD) were immunofluorescently stained with a commercially available polyclonal NFT antibody or a PHF-1 monoclonal antibody, followed by an improved thioflavin-S stain, and finally by Gallyas silver staining. The thioflavin-S method was improved by using a combination quenching method that removes background autofluorescence without remarkable tissue damage and by post-treatment with concentrated phosphate buffer, which minimizes photobleaching. PHF-1 or NFT immunostaining is much less sensitive than the improved thioflavin-S staining and Gallyas silver staining, particularly in the transentorhinal region. Moreover PHF-1 immunoreactivity varied greatly among AD individuals. Thioflavin-S staining and Gallyas silver staining show almost the same sensitivity in NFT demonstration, but only the former depends on the secondary protein structure of NFTs. This study suggests that the improved thioflavin-S staining is a simple, sensitive, and consistent method for demonstration of neurof...Continue Reading

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Citations

Dec 15, 2011·Acta Neuropathologica·Grit TaschenbergerSebastian Kügler
Aug 26, 2003·The American Journal of Pathology·Takashi HoriguchiJohn Q Trojanowski
Oct 14, 2003·Experimental Neurology·Anyang SunGuoying Bing
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Oct 2, 2013·Proceedings of the National Academy of Sciences of the United States of America·Stephanie JanezicRichard Wade-Martins
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