Comparative analysis of axonal transport markers in primary mammalian neurons

Methods in Cell Biology
Eva Klinman, Erika L F Holzbaur

Abstract

Axonal transport is important for neuronal development and the maintenance of effective neuronal function in mature cells. Observing the active transport of organelles and vesicles along the axons of living neurons has emerged as a valuable tool for probing the health of the neuron, and assessing changes associated with stress and neurodegenerative disease. Transport relies on two families of motor proteins: kinesins and dynein. Using these motors, a diverse set of cargos are transported toward the axon tip, the cell body, or anywhere in between. Of particular interest are organelles and cargos associated with disease and the changes in motility that these cargos undergo during pathogenesis. Here, we describe the factors that should be considered when studying different cargos, and the imaging parameters associated with optimal tracking of various organelles and proteins. Ultimately, the ideal cargo to investigate depends on the question being asked and the limitations of individual microscopes available for imaging.

Citations

Feb 9, 2018·Nature·Linas UrnaviciusAndrew P Carter
Jan 15, 2020·Journal of Cell Science·Satish BodakuntlaMaria M Magiera
Jun 13, 2020·Current Protocols in Cell Biology·Himanish BasuThomas L Schwarz
Jun 23, 2020·Current Protocols in Cell Biology·Himanish Basu, Thomas L Schwarz
Apr 18, 2018·Nature Reviews. Molecular Cell Biology·Samara L Reck-PetersonAndrew P Carter
Mar 7, 2019·Frontiers in Cell and Developmental Biology·Valérie BercierCéline Revenu
Apr 10, 2021·Virology·Grayson DuRaine, David C Johnson

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