Comparative analysis of CD8+ T cell responses against human cytomegalovirus proteins pp65 and immediate early 1 shows similarities in precursor frequency, oligoclonality, and phenotype

The Journal of Infectious Diseases
Naeem KhanPaul A H Moss

Abstract

CD8+ T cells are key effectors of the immune response against human cytomegalovirus (HCMV). A number of HCMV-derived CD8+ T cell epitopes are known. Using epitope prediction and subsequent testing for interferon-gamma responses by the ELISPOT assay, we identified an optimal human leukocyte antigen (HLA)-A*0201-restricted CD8+ T cell epitope derived from the major immediate early 1 (IE-1) gene product. As many as one-third of HLA-A*0201-positive, HCMV-seropositive donors make responses to this peptide (residues 316-324 [VLEETSVML]), which can exceed responses against a published immunodominant pp65 epitope (residues 495-503 [NLVPMVATV]). Major histocompatibility complex peptide tetramer staining facilitated detailed phenotypic analyses and revealed populations that resemble terminally differentiated effector cells (CD57+ and CD28-), with considerable restriction in T cell receptor beta-chain variable region use. The results confirm that, although pp65 is a major target for CD8+ T cells, the IE-1 protein may itself stimulate comparable responses in some persons.

Citations

Feb 28, 2008·Medical Microbiology and Immunology·Edward C P WallerMark R Wills
Apr 10, 2004·The International Journal of Biochemistry & Cell Biology·Karl S Peggs, Stephen Mackinnon
Oct 15, 2003·Blood Reviews·Maher K GandhiAndrew J Carmichael
Jan 4, 2012·Cell Research·Hanjie LiJiahuai Han
Feb 9, 2011·Immunology and Cell Biology·John J MilesDavid A Price
Feb 28, 2008·Nature Reviews. Immunology·Vanessa VenturiMiles P Davenport
Dec 6, 2006·Immunology and Cell Biology·Anthony A ScalzoMariapia A Degli-Esposti
Apr 19, 2012·Clinical Obstetrics and Gynecology·Julie JohnsonRobert F Pass
Jan 13, 2009·Clinical Microbiology Reviews·Tania Crough, Rajiv Khanna
Apr 13, 2012·Journal of Virology·Bornali BhattacharjeeTimothy F Kowalik
Feb 14, 2004·Journal of Virology·Urs KarrerPaul Klenerman
Aug 22, 2002·The Journal of Clinical Investigation·David M KoelleLawrence Corey
Aug 20, 2005·The Journal of Clinical Investigation·Andrew D HislopAlan B Rickinson
Dec 15, 2010·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Karl S PeggsMark W Lowdell
Jan 18, 2011·Hematology/oncology Clinics of North America·Per LjungmanMichael Boeckh
Nov 9, 2010·Virus Research·Sarah E JacksonMark R Wills
May 15, 2010·Infectious Disease Clinics of North America·Per LjungmanMichael Boeckh
Jan 6, 2007·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·Christian SinzgerGerhard Jahn
Jan 15, 2008·Journal of Clinical Periodontology·Jamal M SteinStefan Reichert
Feb 1, 2013·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·C Smith, R Khanna
Feb 8, 2005·Human Immunology·Corinna La RosaDon J Diamond
Nov 30, 2004·The Lancet Infectious Diseases·Maher K Gandhi, Rajiv Khanna
Nov 24, 2004·Human Immunology·Brigitte GodardEric Tartour
Jun 3, 2004·Human Immunology·Paul Moss, Naeen Khan

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