Comparative analysis of cell lineage differentiation during hepatogenesis in humans and mice at the single-cell transcriptome level.

Cell Research
Xin WangCheng-Ran Xu

Abstract

During embryogenesis, the liver is the site of hepatogenesis and hematopoiesis and contains many cell lineages derived from the endoderm and mesoderm. However, the characteristics and developmental programs of many of these cell lineages remain unclear, especially in humans. Here, we performed single-cell RNA sequencing of whole human and mouse fetal livers throughout development. We identified four cell lineage families of endoderm-derived, erythroid, non-erythroid hematopoietic, and mesoderm-derived non-hematopoietic cells, and defined the developmental pathways of the major cell lineage families. In both humans and mice, we identified novel markers of hepatic lineages and an ID3+ subpopulation of hepatoblasts as well as verified that hepatoblast differentiation follows the "default-directed" model. Additionally, we found that human but not mouse fetal hepatocytes display heterogeneity associated with expression of metabolism-related genes. We described the developmental process of erythroid progenitor cells during human and mouse hematopoiesis. Moreover, despite the general conservation of cell differentiation programs between species, we observed different cell lineage compositions during hematopoiesis in the human and mous...Continue Reading

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Citations

Sep 4, 2021·Development·Stephanie A CampbellPamela A Hoodless
Aug 31, 2021·Biochemistry and Biophysics Reports·Anyelo DuránAndrés D Klein
Apr 6, 2021·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Shan-Shan WangBo O Zhou

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Methods Mentioned

BETA
scRNA-seq
transgenic
Smart-seq2
PCA
PCR
FACS

Software Mentioned

samtools
Monocle3
princurve
Scanorama
R package Monocle3
velocyto
Ensembl
seq2
Smart
CellCycleScoring

Related Concepts

Related Feeds

CZI Human Cell Atlas Seed Network

The aim of the Human Cell Atlas (HCA) is to build reference maps of all human cells in order to enhance our understanding of health and disease. The Seed Networks for the HCA project aims to bring together collaborators with different areas of expertise in order to facilitate the development of the HCA. Find the latest research from members of the HCA Seed Networks here.

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