Comparative analysis of equine lymphocyte subsets in whole blood and gradient-purified samples

Veterinary Immunology and Immunopathology
M K AkensV Bracher

Abstract

In the present study, two methods of lymphocyte preparation, whole blood lysis and Ficoll-Paque separation, prior to FACS analysis were compared. The comparison was done with single and dual-colour staining techniques. Monoclonal antibodies (mAb) against eCD4, eCD5, eCD8 and eMHC class II were used. There was no significant difference in the results obtained by these two methods.

Citations

Sep 15, 2001·Veterinary Immunology and Immunopathology·M FaldynaM Toman
Sep 5, 2002·Veterinary Immunology and Immunopathology·Claudia ZizzadoroPaolo Ormas
Apr 14, 2004·Journal of Zoo and Wildlife Medicine : Official Publication of the American Association of Zoo Veterinarians·Karen A TerioMichael B Worley
Apr 10, 2002·American Journal of Veterinary Research·Roger SmithRonald J Martens
Sep 4, 2009·The Veterinary Journal·Lutz S GoehringMarianne M Sloet van Oldruitenborgh-Oosterbaan
Nov 26, 2010·Veterinary Microbiology·S WilstermanL S Goehring
Dec 21, 2016·PloS One·Farhid HemmatzadehMajid Kharazian Moghadam

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