Comparative analysis of field-isolate and monkey-adapted Plasmodium vivax genomes

PLoS Neglected Tropical Diseases
Ernest R ChanDavid Serre

Abstract

Significant insights into the biology of Plasmodium vivax have been gained from the ability to successfully adapt human infections to non-human primates. P. vivax strains grown in monkeys serve as a renewable source of parasites for in vitro and ex vivo experimental studies and functional assays, or for studying in vivo the relapse characteristics, mosquito species compatibilities, drug susceptibility profiles or immune responses towards potential vaccine candidates. Despite the importance of these studies, little is known as to how adaptation to a different host species may influence the genome of P. vivax. In addition, it is unclear whether these monkey-adapted strains consist of a single clonal population of parasites or if they retain the multiclonal complexity commonly observed in field isolates. Here we compare the genome sequences of seven P. vivax strains adapted to New World monkeys with those of six human clinical isolates collected directly in the field. We show that the adaptation of P. vivax parasites to monkey hosts, and their subsequent propagation, did not result in significant modifications of their genome sequence and that these monkey-adapted strains recapitulate the genomic diversity of field isolates. Our a...Continue Reading

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Citations

Mar 17, 2016·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Patrick L SuttonSteven A Sullivan
Feb 24, 2018·Frontiers in Cellular and Infection Microbiology·Catarina BourgardFabio T M Costa
Jun 4, 2019·PLoS Neglected Tropical Diseases·Lisa H VerzierJulian C Rayner
Dec 29, 2015·PLoS Neglected Tropical Diseases·David J WinterAnanias A Escalante
Feb 23, 2020·Expert Review of Vaccines·Manuel Alfonso PatarroyoDarwin A Moreno-Pérez
Nov 17, 2020·Frontiers in Cellular and Infection Microbiology·Xin-Zhuan SuDeirdre A Joy
Nov 21, 2017·Trends in Parasitology·Jennifer S Armistead, John H Adams

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Methods Mentioned

BETA
genotyping

Software Mentioned

bowtie2
samtools mpileup

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