Abstract
Bacillus thuringiensis is a widely used biopesticide that produced various insecticidal active substances during its life cycle. Separation and purification of numerous insecticide active substances have been difficult because of the relatively short half-life of such substances. On the other hand, substances can be synthetized at different times during development, so samples at different stages have to be studied, further complicating the analysis. A dual genomic and proteomic approach would enhance our ability to identify such substances, and particularily using mass spectrometry-based proteomic methods. The comparative analysis for genomic and proteomic data have showed that not all of the products deduced from the annotated genome could be identified among the proteomic data. For instance, genome annotation results showed that 39 coding sequences in the whole genome were related to insect pathogenicity, including five cry genes. However, Cry2Ab, Cry1Ia, Cytotoxin K, Bacteriocin, Exoenzyme C3 and Alveolysin could not be detected in the proteomic data obtained. The sporulation-related proteins were also compared analysis, results showed that the great majority sporulation-related proteins can be detected by mass spectrometry...Continue Reading
References
Oct 6, 1989·Science·J B FennC M Whitehouse
Jan 1, 1988·Methods in Enzymology·M S Bergdoll
Jan 1, 1988·Proteins·M M Teeter, M Whitlow
Mar 1, 1984·Infection and Immunity·N E ThompsonE J Schantz
Jan 1, 1993·Memórias do Instituto Oswaldo Cruz·T V GuaycurusL Rabinovitch
Mar 1, 1997·Applied and Environmental Microbiology·S I AsanoT Yamamoto
Aug 26, 1998·FEMS Microbiology Letters·B GeB A Federici
Feb 15, 2001·JAMA : the Journal of the American Medical Association·F S Collins, V A McKusick
May 5, 2001·Trends in Microbiology·D A Relman, S Falkow
Nov 24, 2001·Trends in Genetics : TIG·S Peri, A Pandey
Jan 1, 2003·Journal of Molecular Biology·Bogdan Polevoda, Fred Sherman
Jan 20, 2004·Proteomics·Jacob D JaffeGeorge M Church
May 14, 2004·Annual Review of Biophysics and Biomolecular Structure·John R Yates
Aug 4, 2006·Applied and Environmental Microbiology·Takeshi ItoShin-ichiro Asano
Mar 6, 2007·Journal of Bacteriology·Jean F ChallacombeThomas S Brettin
Jul 26, 2007·Chemical Reviews·Tao LiuRichard D Smith
Apr 23, 2008·Genome Research·Nitin GuptaPavel A Pevzner
Aug 23, 2008·Current Opinion in Chemical Biology·Xuemei HanJohn R Yates
Mar 20, 2010·Genome Research·Nadine BorchertBoris Macek
Jun 8, 2010·Journal of Bacteriology·Jin HeZiniu Yu
Jan 20, 2011·Proteomics·Santosh RenuseAkhilesh Pandey
May 10, 2011·Journal of Bacteriology·Jin HeZiniu Yu
Jun 2, 2011·Proteomics·Dandan WuZiniu Yu
May 29, 2012·Applied and Environmental Microbiology·Shaoya HuangLiqiu Xia
Mar 22, 2013·Genome Announcements·Guiming LiuFuping Song
Mar 22, 2013·Genome Announcements·Emilia MurawskaIzabela Swiecicka
Dec 7, 2013·Genome Announcements·Norman A DoggettCliff S Han
Citations
Jun 16, 2016·Frontiers in Microbiology·Meifang QuanXuezhi Ding
Nov 16, 2018·Proteomics·Teck Yew LowRahman Jamal
Sep 30, 2019·Current Microbiology·Nancy FayadMireille Kallassy Awad
Nov 28, 2017·Applied Microbiology and Biotechnology·Bhupendra S PanwarSarvjeet Kaur
Aug 28, 2020·Frontiers in Microbiology·Meifang QuanXuezhi Ding
Oct 7, 2015·Microbial Cell Factories·Xuemei LiuXuezhi Ding
May 12, 2018·Toxins·Joaquín Gomis-CebollaJuan Ferré
Sep 26, 2019·Frontiers in Microbiology·Junyan XieXuezhi Ding
Oct 30, 2020·Toxins·Ayda KhorramnejadBaltasar Escriche
Mar 7, 2021·International Journal of Molecular Sciences·Anton E ShikovKirill S Antonets