Comparative analysis of genomics and proteomics in Bacillus thuringiensis 4.0718

PloS One
Jie RangLiqiu Xia

Abstract

Bacillus thuringiensis is a widely used biopesticide that produced various insecticidal active substances during its life cycle. Separation and purification of numerous insecticide active substances have been difficult because of the relatively short half-life of such substances. On the other hand, substances can be synthetized at different times during development, so samples at different stages have to be studied, further complicating the analysis. A dual genomic and proteomic approach would enhance our ability to identify such substances, and particularily using mass spectrometry-based proteomic methods. The comparative analysis for genomic and proteomic data have showed that not all of the products deduced from the annotated genome could be identified among the proteomic data. For instance, genome annotation results showed that 39 coding sequences in the whole genome were related to insect pathogenicity, including five cry genes. However, Cry2Ab, Cry1Ia, Cytotoxin K, Bacteriocin, Exoenzyme C3 and Alveolysin could not be detected in the proteomic data obtained. The sporulation-related proteins were also compared analysis, results showed that the great majority sporulation-related proteins can be detected by mass spectrometry...Continue Reading

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Citations

Nov 28, 2017·Applied Microbiology and Biotechnology·Bhupendra S PanwarSarvjeet Kaur

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Methods Mentioned

BETA
electrophoresis
PCR
acetylation

Software Mentioned

Hiseq
DNAman
SOAPdenovo
- SE
WebMGA
BLAST
Notes
SE -
- SE -
Gene Construction Kit

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