Comparative analysis of HER2 copy number between plasma and tissue samples in gastric cancer using droplet digital PCR.

Scientific Reports
Boram KimHye Seung Lee

Abstract

In this study, we measured the human epidermal growth factor receptor 2 (HER2) copy number in both tissue and plasma samples of gastric cancer patients by using droplet digital polymerase chain reaction (ddPCR) method. Eighty gastric cancer patients were enrolled and both formalin-fixed and paraffin-embedded tissue and preoperative plasma samples were collected. HER2 status was determined by HER2 immunohistochemistry (IHC)/silver in situ hybridization (SISH) in tissue samples and ddPCR of the target gene HER2 and the reference gene eukaryotic translation initiation factor 2C, 1 in both tissue and plasma. The concordance rate of tissue HER2 status determined by IHC/SISH and HER2 ddPCR was 90.0% (72/80), and the sensitivity and specificity of tissue ddPCR were 85.0% and 95.0%, respectively. The concordance rate of plasma ddPCR and IHC/SISH was 63.8% (51/80). The sensitivity, specificity, positive predictive value, and negative predictive value of plasma HER2 ddPCR were 37.5%, 90.0%, 79.0%, and 59.0%, respectively. As HER2 measurement by tissue ddPCR showed a high concordance rate with HER2 status by IHC/SISH, it could replace tissue IHC/SISH testing in gastric cancer. These findings may contribute to the development of tissue and...Continue Reading

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Citations

Dec 12, 2020·Expert Review of Molecular Diagnostics·Andrea Moreno-ManuelEloisa Jantus-Lewintre
Feb 6, 2021·Journal of Cancer Research and Clinical Oncology·Zimin LiuLu Zhang
May 13, 2021·Best Practice & Research. Clinical Gastroenterology·Mārcis Leja, Aija Linē

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Methods Mentioned

BETA
PCR
biopsy

Software Mentioned

SPSS
QuantaSoft

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