Comparative analysis of immunological reconstitution induced by vascularized bone marrow versus bone marrow cell transplantation

Transplant International : Official Journal of the European Society for Organ Transplantation
B LukomskaW L Olszewski

Abstract

We have reported previously that vascularized bone marrow transplantation (VBMT) in an orthotopic hind limb graft brings about complete repopulation of bone marrow cavities in lethally irradiated syngeneic recipients within 10 days. Intravenous infusion of an equivalent volume of bone marrow cell suspension was evidently less effective. The purpose of this study was to investigate the reconstitution of immunocompetent compartments of lethally irradiated syngeneic rats after VBMT. Lewis rat hind limbs were transplanted orthotopically into irradiated recipients. Ten days after irradiation and bone marrow transplantation, bone marrow, mesenteric lymph nodes, and sera from rats were harvested. Mesenteric lymph node lymphocytes were analyzed. The responsiveness for mesenteric lymph node lymphocytes (MLNL) to mitogens and cell proliferation in the presence of sera and bone marrow cell (BMC) culture supernatants were measured. Our studies have shown that vascularized bone marrow transplantation brings about rapid replenishment of lymphoid organs of lethally irradiated syngeneic recipients. The repopulating subsets are fully responsive to mitogens. Sera from reconstituting rats had no effect on the proliferation of mature lymphocytes. ...Continue Reading

References

Aug 16, 1994·Proceedings of the National Academy of Sciences of the United States of America·T R KollmannH Goldstein

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Citations

Jan 1, 1993·Transplant Immunology·J StorekL Goldstein
Mar 26, 2008·Annals of Plastic Surgery·Maria Siemionow, Serdar Nasir
Nov 18, 2009·Transplantation Proceedings·K V RavindraS T Ildstad
Jul 1, 2008·Transplantation Proceedings·K V RavindraS T Ildstad
May 1, 2008·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·M SiemionowK Carnevale
Sep 2, 2009·Transplant International : Official Journal of the European Society for Organ Transplantation·Maria Siemionow, Aleksandra Klimczak
Apr 11, 2018·Plastic and Reconstructive Surgery. Global Open·Byoungchol OhAmir H Dorafshar
Aug 8, 2006·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·O ThaunatE Morelon
Jun 29, 2007·Microsurgery·Maria Siemionow, Serdar Nasir
Mar 2, 2006·Annals of Plastic Surgery·Selahattin OzmenMaria Siemionow

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