Comparative analysis of metagenomes from three methanogenic hydrocarbon-degrading enrichment cultures with 41 environmental samples

The ISME Journal
Boonfei TanLisa M Gieg

Abstract

Methanogenic hydrocarbon metabolism is a key process in subsurface oil reservoirs and hydrocarbon-contaminated environments and thus warrants greater understanding to improve current technologies for fossil fuel extraction and bioremediation. In this study, three hydrocarbon-degrading methanogenic cultures established from two geographically distinct environments and incubated with different hydrocarbon substrates (added as single hydrocarbons or as mixtures) were subjected to metagenomic and 16S rRNA gene pyrosequencing to test whether these differences affect the genetic potential and composition of the communities. Enrichment of different putative hydrocarbon-degrading bacteria in each culture appeared to be substrate dependent, though all cultures contained both acetate- and H2-utilizing methanogens. Despite differing hydrocarbon substrates and inoculum sources, all three cultures harbored genes for hydrocarbon activation by fumarate addition (bssA, assA, nmsA) and carboxylation (abcA, ancA), along with those for associated downstream pathways (bbs, bcr, bam), though the cultures incubated with hydrocarbon mixtures contained a broader diversity of fumarate addition genes. A comparative metagenomic analysis of the three cult...Continue Reading

References

Feb 1, 1997·Journal of Bacteriology·H R Beller, A M Spormann
Jan 27, 1999·Bioinformatics·S R Eddy
Dec 3, 1999·Applied and Environmental Microbiology·M FickerE Edwards
Aug 31, 2000·Journal of Molecular Biology·C NotredameJ Heringa
Dec 1, 2000·Applied and Environmental Microbiology·H R Beller, E A Edwards
Apr 11, 2001·The Journal of Biological Chemistry·O Kniemeyer, J Heider
Feb 1, 2003·Environmental Microbiology·Ania C Ulrich, Elizabeth A Edwards
Oct 8, 2003·Systematic Biology·Stéphane Guindon, Olivier Gascuel
Dec 6, 2003·Environmental Science & Technology·G Todd TownsendJoseph M Suflita
Mar 23, 2004·Nucleic Acids Research·Robert C Edgar
Apr 20, 2004·Journal of Bacteriology·Silvia G AcinasMartin F Polz
Sep 27, 2006·Environmental Science & Technology·Tariq SiddiqueJulia M Foght
Dec 5, 2006·Applied and Environmental Microbiology·Cheryl E Washer, Elizabeth A Edwards
Apr 19, 2007·Environmental Science & Technology·Tariq SiddiqueJulia M Foght
Aug 8, 2008·Journal of Molecular Microbiology and Biotechnology·Julia Foght
Mar 5, 2009·Microbiology and Molecular Biology Reviews : MMBR·Manuel CarmonaEduardo Díaz
May 7, 2009·FEMS Microbiology Ecology·Nidal Abu LabanRainer U Meckenstock
Jul 8, 2009·Journal of Bacteriology·Christopher B WalkerDavid A Stahl
Nov 17, 2009·Journal of Bioscience and Bioengineering·Nahoko SakaiKazuo Yamamoto
Nov 19, 2009·Nucleic Acids Research·Robert D FinnAlex Bateman
Dec 18, 2009·Ground Water·Hedeff I EssaidGeoffrey N Delin
Jan 22, 2010·The ISME Journal·Anna EngelbrektsonPhilip Hugenholtz
Feb 5, 2010·Bioinformatics·Donovan H Parks, Robert G Beiko
Aug 6, 2010·The ISME Journal·Athanasios LykidisWen-Tso Liu
Dec 7, 2010·Environmental Science & Technology·Esther Ramos-PadrónLisa M Gieg
May 25, 2011·International Journal of Cardiology·Rebecca KozorGemma A Figtree
Sep 2, 2011·Environmental Microbiology·Ralf RabusHeinz Wilkes
Nov 2, 2011·Environmental Microbiology·S Jane FowlerLisa M Gieg
Dec 8, 2011·FEMS Microbiology Ecology·Boris WawrikAmy V Callaghan
Dec 14, 2011·Applied and Environmental Microbiology·Weimin Sun, Alison M Cupples
Feb 14, 2012·FEMS Microbiology Ecology·Carolina Berdugo-ClavijoLisa M Gieg

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Citations

Jan 7, 2016·Frontiers in Microbiology·Antje GittelKasper U Kjeldsen
Nov 19, 2015·Genome Génome / Conseil National De Recherches Canada·Julia M Foght
Oct 7, 2016·Frontiers in Microbiology·Jayeeta SarkarPinaki Sar
Mar 24, 2017·FEMS Microbiology Ecology·Julia M FoghtTariq Siddique
May 6, 2017·Scientific Reports·Atsushi KouzumaKazuya Watanabe
Sep 27, 2020·International Journal of Environmental Research and Public Health·Eduardo Jahir GutiérrezDavid Tirado
May 30, 2017·Frontiers in Microbiology·Wael A IsmailJi-Dong Gu
May 18, 2020·Scientific Reports·Glenn D ChristmanJennifer F Biddle
Aug 26, 2020·Nature Microbiology·Lin-Xing ChenJillian F Banfield
Nov 26, 2019·Ecotoxicology and Environmental Safety·Sanderson Tarciso Pereira de SousaValéria Maia de Oliveira
Dec 12, 2020·Frontiers in Microbiology·Krisztián LacziKatalin Perei
Jan 8, 2021·Environmental Science and Pollution Research International·Nishi Kumari Singh, Sangeeta Choudhary
May 5, 2021·Environmental Science and Pollution Research International·Siddhartha PalSufia K Kazy
Feb 15, 2019·The Science of the Total Environment·Xiaotong WangYanming Zhang

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