Comparative analysis of mouse and human preimplantation development following POU5F1 CRISPR/Cas9 targeting reveals interspecies differences.

Human Reproduction
P StamatiadisB Heindryckx

Abstract

What is the role of POU class 5 homeobox 1 (POU5F1) in human preimplantation development and how does it compare with the mouse model? POU5F1 is required for successful development of mouse and human embryos to the blastocyst stage as knockout embryos exhibited a significantly lower blastocyst formation rate, accompanied by lack of inner cell mass (ICM) formation. Clustered regularly interspaced short palindromic repeats-CRISPR associated genes (CRISPR-Cas9) has previously been used to examine the role of POU5F1 during human preimplantation development. The reported POU5F1-targeted blastocysts always retained POU5F1 expression in at least one cell, because of incomplete CRISPR-Cas9 editing. The question remains of whether the inability to obtain fully edited POU5F1-targeted blastocysts in human results from incomplete editing or the actual inability of these embryos to reach the blastocyst stage. The efficiency of CRISPR-Cas9 to induce targeted gene mutations was first optimized in the mouse model. Two CRISPR-Cas9 delivery methods were compared in the B6D2F1 strain: S-phase injection (zygote stage) (n = 135) versus metaphase II-phase (M-phase) injection (oocyte stage) (n = 23). Four control groups were included: non-injected me...Continue Reading

References

Mar 29, 1990·Nature·Hans R SchölerP Gruss
Nov 1, 1991·Mechanisms of Development·Y I YeomK Artzt
Dec 11, 1999·Cells, Tissues, Organs·M PesceHans R Schöler
Sep 5, 2002·Biochemical and Biophysical Research Communications·Mi-Hyun KimFuyuki Ishikawa
Sep 23, 2003·Genesis : the Journal of Genetics and Development·J Matthew Velkey, K Sue O'Shea
May 27, 2004·Cytogenetic and Genome Research·G IliakisG Pantelias
Nov 24, 2004·The Journal of Biological Chemistry·Sayaka Okumura-NakanishiFuyuki Ishikawa
Apr 30, 2005·The Journal of Biological Chemistry·David J RoddaPaul Robson
Sep 13, 2005·Cell·Laurie A BoyerRichard A Young
Mar 19, 2011·Cell·Richard A Young
Aug 13, 2013·Nature Cell Biology·Guangming WuHans R Schöler
Dec 29, 2013·Trends in Cell Biology·Aliaksandra Radzisheuskaya, José C R Silva
Oct 31, 2014·Nature Protocols·Ivan BedzhovMagdalena Zernicka-Goetz
Jul 28, 2016·Scientific Reports·Annekatrien BoelAndy Willaert
Aug 9, 2016·Reproduction : the Official Journal of the Society for the Study of Fertility·Atsushi FukudaHidenori Akutsu
Aug 8, 2017·Nature·Hong MaShoukhrat Mitalipov
Sep 28, 2017·Nature·N. M. E. FogartyKathy K Niakan
Oct 5, 2017·Nature·N. M. E. FogartyKathy K Niakan
May 19, 2018·Scientific Reports·Bradford W DaigneaultPablo J Ross
Oct 19, 2019·Scientific Reports·I Lamas-ToranzoP Bermejo-Álvarez
Oct 31, 2020·Cell·Michael V ZuccaroDieter Egli

Related Concepts

Down-Regulation
Embryo
Embryonic Development
Exons
Gene Clusters
Genes
Hospitals
Laboratory mice
Microinjections
Oocytes

Related Feeds

CRISPR (general)

Clustered regularly interspaced short palindromic repeats (CRISPR) are DNA sequences in the genome that are recognized and cleaved by CRISPR-associated proteins (Cas). CRISPR-Cas system enables the editing of genes to create or correct mutations. Discover the latest research on CRISPR here.

CRISPR Ribonucleases Deactivation

CRISPR-Cas system enables the editing of genes to create or correct mutations. This feed focuses on mechanisms that underlie deactivation of CRISPR ribonucleases. Here is the latest research.

CRISPR for Genome Editing

Genome editing technologies enable the editing of genes to create or correct mutations. Clustered regularly interspaced short palindromic repeats (CRISPR) are DNA sequences in the genome that are recognized and cleaved by CRISPR-associated proteins (Cas). Here is the latest research on the use of CRISPR-Cas system in gene editing.