Apr 14, 2018

Comparative analysis of naive, primed and ground state pluripotency in mouse embryonic stem cells originating from the same genetic background

Scientific Reports
Sabitri GhimireBjörn Heindryckx

Abstract

Mouse embryonic stem cells (mESCs) exist in a naive, primed and ground state of pluripotency. While comparative analyses of these pluripotency states have been reported, the mESCs utilized originated from various genetic backgrounds and were derived in different laboratories. mESC derivation in conventional LIF + serum culture conditions is strain dependent, with different genetic backgrounds potentially affecting subsequent stem cell characteristics. In the present study, we performed a comprehensive characterization of naive, primed and ground state mESCs originating from the same genetic background within our laboratory, by comparing their transcriptional profiles. We showed unique transcriptional profiles for naive, primed and ground state mESCs. While naive and ground state mESCs have more similar but not identical profiles, primed state mESCs show a very distinct profile. We further demonstrate that the differentiation propensity of mESCs to specific germ layers is highly dependent on their respective state of pluripotency.

  • References23
  • Citations7

Mentioned in this Paper

Study
HMGB Proteins
Fibroblast growth factor-5
Pluripotent Stem Cells
Transcription, Genetic
Pou5f1 protein, mouse
Nanog Homeobox Protein
Fgf5 protein, mouse
Sox17 protein, mouse
Dicom Derivation

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