Comparative analysis of neuroectodermal differentiation capacity of human bone marrow stromal cells using various conversion protocols

Journal of Neuroscience Research
Andreas HermannAlexander Storch

Abstract

Human adult bone marrow-derived mesodermal stromal cells (hMSCs) are able to differentiate into multiple mesodermal tissues, including bone and cartilage. There is evidence that these cells are able to break germ layer commitment and differentiate into cells expressing neuroectodermal properties. There is still debate about whether this results from cell fusion, aberrant marker gene expression or real neuroectodermal differentiation. Here we extend our work on neuroectodermal conversion of adult hMSCs in vitro by evaluating various epigenetic conversion protocols using quantitative RT-PCR and immunocytochemistry. Undifferentiated hMSCs expressed high levels of fibronectin as well as several neuroectodermal genes commonly used to characterize neural cell types, such as nestin, beta-tubulin III, and GFAP, suggesting that hMSCs retain the ability to differentiate into neuroectodermal cell types. Protocols using a direct differentiation of hMSCs into a neural phenotype failed to induce significant changes in morphology and/or expression of markers of early and mature glial/neuronal cells types. In contrast, a multistep protocol with conversion of hMSCs into a neural stem cell-like population and subsequent terminal differentiation ...Continue Reading

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Citations

Jul 4, 2013·Der Nervenarzt·A HermannS Liebau
May 31, 2013·Journal of Neural Transmission·Andreas Hermann, Alexander Storch
Jan 1, 2008·Transfusion Medicine and Hemotherapy : Offizielles Organ Der Deutschen Gesellschaft Fur̈ Transfusionsmedizin Und Immunham̈atologie·Jeffrey M GimbleBruce A Bunnell
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Nov 21, 2012·Regenerative Medicine·M Beatriz Durán AlonsoThomas Schimmang
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Apr 10, 2010·Journal of Biomedicine & Biotechnology·Igor TitushkinMichael Cho
Jul 26, 2011·Acta Biomaterialia·A NairL Tang
Jan 25, 2011·Translational Research : the Journal of Laboratory and Clinical Medicine·Meghan G ThomasLivia Hool
Apr 28, 2009·Differentiation; Research in Biological Diversity·Florian VöllnerChristian Morsczeck
Sep 8, 2009·Journal of the Neurological Sciences·Anne K MeyerAlexander Storch
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Dec 14, 2011·Annals of Neurology·Luigi TitomanlioPierre Gressens
Jan 22, 2014·Cell Biology International·Fereshteh AzediNiknam Lakpour

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