Comparative Analysis of Non-viral Transfection Methods in Mouse Embryonic Fibroblast Cells

Journal of Biomolecular Techniques : JBT
Migi LeeIsabel Dominguez

Abstract

Mouse embryonic fibroblast (MEF) cells are an important in vitro model for developmental biology, disease, and reprogramming studies. However, as with other primary cells, they are challenging to transfect. Although viral gene-delivery methods achieve high gene-delivery efficiency, challenges with cell mutagenesis and safety among others have led to the use and improvement of non-viral gene-delivery methods in MEF cells. Despite the importance of gene delivery in MEF cells, there is limited comparison of method/reagent efficacy. In this study, we compared the effectiveness of different gene-delivery methods and several reagents currently available in MEF cells by introducing a plasmid containing enhanced green fluorescent protein (EGFP). We analyze transfection efficiency by EGFP fluorescence. Our results suggest that two gene-delivery methods-electroporation and magnetofection in combination with a lipid reagent, are the most efficient transfection methods in MEF cells. This study provides a foundation for the selection of transfection methods or reagents when using MEF cells.

Citations

Apr 17, 2019·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·Yue LiJinghua Liu
Aug 3, 2019·Scientific Reports·Rupali WaliaHermann M Schatzl
Oct 3, 2020·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Yana TarakanchikovaDmitry Gorin
Sep 12, 2018·Journal of Biophotonics·Timofey PylaevNikolai Khlebtsov
Oct 10, 2018·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Tao WangRakesh N Veedu
Jul 10, 2018·Nature Communications·Elena SenísDirk Grimm
May 8, 2020·Advanced Materials·Yaping ChenNicolas H Voelcker
Feb 18, 2021·Advanced Materials·Dorsa Morshedi RadMajid Ebrahimi Warkiani
Mar 29, 2021·Biotechnology Progress·Mirosław KucharskiEwa Ocłoń

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