Comparative analysis of primary tumour and matched metastases in colorectal cancer patients: evaluation of concordance between genomic and transcriptional profiles

European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)
Stéphane VignotJean-Philippe Spano

Abstract

Focal and temporal tumour heterogeneity can represent a major challenge for biology-guided therapies. This study proposes to investigative molecular discrepancies between primary colorectal cancer (CRC) samples and matched metastases. Surgical samples from primary and matched metastatic tissues from 13 CRC patients along with their adjacent normal tissue were evaluated. A mutational analysis was performed using a targeted Next Generation Sequencing assay (Foundation Medicine) with a focus on known recurrent somatic mutations as surrogate of key oncogenic events. Gene expression analysis was also performed to investigate transcriptional discrepancies. Among the 26 samples, 191 mutations were identified including mutations in APC (13 pts), TP53 (11 pts), and KRAS (7 pts). Global concordance rate for mutations was 78% between primary and metastatic tumours and raised to 90% for 12 known recurrent mutations in CRC. Differential gene expression analysis revealed a low number of significantly variant transcripts between primary and metastatic tumours once the tissue effect was taken into account. Only two pathways (ST_ADRENERGIC, PID_REELINPATHWAY) were differentially up-regulated in metastases among 17 variant pathways. A common pro...Continue Reading

References

Dec 15, 1993·Biochemical and Biophysical Research Communications·J J HouriP Codogno
Oct 4, 2005·Proceedings of the National Academy of Sciences of the United States of America·Aravind SubramanianJill P Mesirov
May 2, 2006·Statistical Applications in Genetics and Molecular Biology·Gordon K Smyth
Aug 8, 2006·Nature Medicine·Larry Norton, Joan Massagué
Jul 22, 2008·Biochemical and Biophysical Research Communications·William Ka Kei WuChi Hin Cho
Apr 22, 2009·Cancer Genetics and Cytogenetics·Herui YaoYufen Xie
May 20, 2009·Bioinformatics·Heng Li, Richard Durbin
Jun 10, 2009·Bioinformatics·Heng LiUNKNOWN 1000 Genome Project Data Processing Subgroup
Jan 13, 2010·Cell·Mi-Young KimJoan Massagué
Oct 19, 2010·Nucleic Acids Research·Simon A ForbesP Andrew Futreal
Mar 8, 2011·Cell·Douglas Hanahan, Robert A Weinberg
May 14, 2011·Life Sciences·Helen P S WongChi Hin Cho
Jun 3, 2011·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Amal Melhem-BertrandtAna-Maria Gonzalez-Angulo
Jun 6, 2012·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Efsevia VakianiDavid B Solit
Jun 20, 2012·Critical Reviews in Oncology/hematology·Stéphane VignotJean-Charles Soria
Sep 25, 2012·Cancer Research·Charles Swanton
Mar 30, 2013·Science·Bert VogelsteinKenneth W Kinzler
May 1, 2013·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Stéphane VignotElisabeth Brambilla
May 4, 2013·Experimental Cell Research·Anna-Maria StockFrank Entschladen

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Citations

Jun 18, 2015·International Journal of Molecular Sciences·Moisés Blanco-CalvoManuel Valladares-Ayerbes
Mar 5, 2016·Cancer Metastasis Reviews·Aleš Ryška
Mar 8, 2016·Cancer Metastasis Reviews·Adriana AmaroUlrich Pfeffer
Apr 29, 2016·Science·Samra Turajlic, Charles Swanton
Oct 16, 2016·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Elvira Khialeeva, Ellen M Carpenter
May 4, 2017·Expert Review of Gastroenterology & Hepatology·Ashton A Connor, Steven Gallinger
Oct 25, 2017·International Journal of Molecular Sciences·Jessica RoelandsWouter Hendrickx
Mar 28, 2017·World Journal of Gastrointestinal Oncology·Torhild Veen, Kjetil Søreide
Sep 8, 2018·EMBO Molecular Medicine·María Virtudes CéspedesRamón Mangues
Apr 10, 2020·International Journal of Cancer. Journal International Du Cancer·Andreas SchlickerLoredana Vecchione
Mar 31, 2016·Oncotarget·François BertucciMax Chaffanet
Mar 30, 2017·Diagnostic Pathology·Kajsa Ericson-LindquistHans Brunnström
Mar 16, 2019·Scientific Reports·Bo Young OhWoong-Yang Park
Sep 29, 2019·World Journal of Gastrointestinal Oncology·Chiaki InagakiTaroh Satoh
Nov 30, 2018·Journal of Experimental & Clinical Cancer Research : CR·Dong-Min ShiWei-Zhong Wu
Feb 26, 2020·British Journal of Cancer·Joanne M MankorMartijn P J K Lolkema
Apr 26, 2020·Cell Death & Disease·Alessandro OttaianoGuglielmo Nasti
Oct 18, 2020·Biomedicines·Ugo TestaElvira Pelosi
Apr 18, 2019·Cancer Discovery·Caroline E McCoach, Trever G Bivona
Sep 25, 2017·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Je-Gun JoungWoo Yong Lee
Mar 5, 2016·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Alexa B SchrockVincent A Miller
Jun 3, 2020·Seminars in Cancer Biology·María González-GonzálezAlberto Orfao
May 26, 2021·Translational Oncology·Alessandro OttaianoGuglielmo Nasti
Jun 3, 2021·International Journal of Molecular Sciences·Seung Eun LeeHye Seung Han
Jul 3, 2021·Brain Sciences·Filippo BiamonteAlessio D'Alessio

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