Comparative analysis of selected exhaled breath biomarkers obtained with two different temperature-controlled devices.

BMC Pulmonary Medicine
Frank HoffmeyerThomas Brüning

Abstract

The collection of exhaled breath condensate (EBC) is a suitable and non-invasive method for evaluation of airway inflammation. Several studies indicate that the composition of the condensate and the recovery of biomarkers are affected by physical characteristics of the condensing device and collecting circumstances. Additionally, there is an apparent influence of the condensing temperature, and often the level of detection of the assay is a limiting factor. The ECoScreen2 device is a new, partly single-use disposable system designed for studying different lung compartments. EBC samples were collected from 16 healthy non-smokers by using the two commercially available devices ECoScreen2 and ECoScreen at a controlled temperature of -20 degrees C. EBC volume, pH, NOx, LTB4, PGE2, 8-isoprostane and cys-LTs were determined. EBC collected with ECoScreen2 was less acidic compared to ECoScreen. ECoScreen2 was superior concerning condensate volume and detection of biomarkers, as more samples were above the detection limit (LTB4 and PGE2) or showed higher concentrations (8-isoprostane). However, NOx was detected only in EBC sampled by ECoScreen. ECoScreen2 in combination with mediator specific enzyme immunoassays may be suitable for meas...Continue Reading

References

Jul 1, 1979·British Journal of Clinical Pharmacology·M Hills, P Armitage
Sep 1, 1995·American Journal of Respiratory and Critical Care Medicine
Jan 10, 1998·British Journal of Anaesthesia·E TroncyG Blaise
Oct 13, 2000·American Journal of Respiratory and Critical Care Medicine·T HanazawaP J Barnes
Sep 4, 2001·Respiratory Medicine·K GanasN Kalogeropoulos
Mar 5, 2002·American Journal of Respiratory and Critical Care Medicine·Richard M EffrosFeng Sun
Apr 10, 2002·The Journal of Allergy and Clinical Immunology·Paolo Montuschi, Peter J Barnes
May 15, 2002·Trends in Pharmacological Sciences·Paolo Montuschi, Peter J Barnes
May 23, 2002·American Journal of Respiratory and Critical Care Medicine·Konstantinos KostikasStelios Loukides
Jul 10, 2002·Biomarkers : Biochemical Indicators of Exposure, Response, and Susceptibility to Chemicals·Sergei A Kharitonov, Peter J Barnes
Nov 19, 2003·The European Respiratory Journal·K KostikasS Loukides
Dec 19, 2003·The European Respiratory Journal·J VaughanJ Hunt
Feb 26, 2004·The European Respiratory Journal·P G A Van HoydonckE H M Temme
Jul 15, 2004·Respiratory Medicine·E BucchioniS A Kharitonov
May 17, 2005·Respiratory Medicine·Ellen Tufvesson, Leif Bjermer
Sep 2, 2005·The European Respiratory Journal·I HorváthUNKNOWN ATS/ERS Task Force on Exhaled Breath Condensate
Sep 3, 2005·BMC Pulmonary Medicine·Matteo GoldoniAntonio Mutti
Nov 18, 2005·Pediatric Pulmonology·Ting F LeungGary W K Wong
Feb 16, 2006·Chest·Alix O Paget-BrownJohn F Hunt
Jul 28, 2006·The European Respiratory Journal·P P RosiasE Dompeling
Nov 3, 2006·The European Respiratory Journal·T KullmannI Horváth
Nov 18, 2006·American Journal of Respiratory and Critical Care Medicine·Abigail S JacksonDeborah H Yates
Mar 10, 2007·Current Medicinal Chemistry·Paolo MontuschiL Jackson Roberts
Oct 2, 2007·The European Respiratory Journal·J LiuP S Thomas
Jan 11, 2008·The European Respiratory Journal·P P RosiasE Dompeling
Jan 25, 2008·The European Respiratory Journal·D L BayleyR A Stockley
Mar 14, 2008·Current Medicinal Chemistry·Angela KoutsokeraKonstantinos Kostikas
Nov 4, 2008·The European Respiratory Journal·E SapeyR Stockley
Jun 18, 2009·Current Opinion in Allergy and Clinical Immunology·Frank HoffmeyerThomas Brüning

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Citations

Dec 15, 2010·International Journal of Molecular Sciences·Monika JanickaJacek Namieśnik
Jun 13, 2012·Journal of Toxicology and Environmental Health. Part a·Frank HoffmeyerUNKNOWN Weldox Group
Nov 14, 2014·Journal of Proteome Research·Andrea MottaMauro Maniscalco
Mar 10, 2011·The Journal of Asthma : Official Journal of the Association for the Care of Asthma·Luis PrietoJulio Marin
Jul 6, 2010·Pulmonary Pharmacology & Therapeutics·Z DiamantR Gerth van Wijk
Mar 9, 2012·Respiratory Medicine·Stewart R CarterElizabeth J Kovacs
Nov 26, 2010·Journal of Environmental Monitoring : JEM·Frank HoffmeyerThomas Brüning
Mar 9, 2011·Journal of Breath Research·Eleanor A ChapmanDeborah H Yates
Apr 28, 2017·The European Respiratory Journal·Ildiko HorváthTeunis J Vink
Apr 24, 2012·Journal of Breath Research·Philippe Rosias
May 15, 2015·Journal of Breath Research·F HoffmeyerJ Bünger
Jul 6, 2016·Journal of Breath Research·Sarah A HayesViive M Howell
Jun 3, 2020·International Journal of Molecular Sciences·Yara ShomanIrina Guseva Canu
May 5, 2017·Scientific Reports·A Peralbo-MolinaF Priego-Capote
Jul 18, 2018·Acta Medica (Hradec Králové)·Eva PeterováJan Bureš
Jul 18, 2018·Analytica Chimica Acta·Konstantin O ZamuruyevCristina E Davis

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Methods Mentioned

BETA
lavage

Software Mentioned

GraphPad Prism
Softmax Pro
MasterLab pro

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