Nov 5, 2018

Comparative analysis of sequencing technologies platforms for single-cell transcriptomics

BioRxiv : the Preprint Server for Biology
Kedar Nath NatarajanSarah Teichmann

Abstract

All single-cell RNA-seq protocols and technologies require library preparation prior to sequencing on a platform such as Illumina. Here, we present the first report to utilize the BGISEQ-500 platform for scRNA-seq, and compare the sensitivity and accuracy to Illumina sequencing. We generate a scRNA-seq resource of 468 unique single-cells and 1,297 matched single cDNA samples, performing SMARTer and Smart-seq2 protocols on mESCs and K562 cells with RNA spike-ins. We sequence these libraries on both BGISEQ-500 and Illumina HiSeq platforms using single- and paired-end reads. The two platforms have comparable sensitivity and accuracy in terms of quantification of gene expression, and low technical variability. Our study provides a standardised scRNA-seq resource to benchmark new scRNA-seq library preparation protocols and sequencing platforms.

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Mentioned in this Paper

Study
Nucleic Acid Sequencing
Sequence Determinations
Gene Expression
Mouse Embryonic Stem Cells
Sequencing
RNA, Small Cytoplasmic
Molecular Genetic Technique
Gene Expression Profiling
Microarray Platform

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