Comparative analysis of space-grown and earth-grown crystals of an aminoacyl-tRNA synthetase: space-grown crystals are more useful for structural determination

Acta Crystallographica. Section D, Biological Crystallography
Joseph D NgR Giegé

Abstract

Protein crystallization under microgravity aims at benefiting from the quasi-absence of convection and sedimentation to favor well ordered crystal nucleation and growth. The dimeric multidomain enzyme aspartyl-tRNA synthetase from Thermus thermophilus has been crystallized within dialysis reactors of the Advanced Protein Crystallization Facility in the laboratory on earth and under microgravity aboard the US Space Shuttle. A strictly comparative crystallographic analysis reveals that the crystals grown in space are superior in every respect to control crystals prepared in otherwise identical conditions on earth. They diffract X-rays more intensely and have a lower mosaicity, facilitating the process of protein structure determination. Indeed, the electron-density map calculated from diffraction data of space-grown crystals contains considerably more detail. The resulting three-dimensional structure model at 2.0 A resolution is more accurate than that produced in parallel using the data originating from earth-grown crystals. The major differences between the structures, including the better defined amino-acid side chains and the higher order of bound water molecules, are emphasized.

Citations

Dec 9, 2003·Microgravity Science and Technology·Huai-Xing CangRu-Chang Bi
Apr 30, 2003·Journal of Structural Biology·David HosfieldRaymond C Stevens
Jan 18, 2013·The Journal of Biological Chemistry·Richard Giegé
Jul 11, 2007·Acta Crystallographica. Section F, Structural Biology and Crystallization Communications·Kaoru SuzukiAkio Takénaka
Oct 15, 2010·Acta Crystallographica. Section F, Structural Biology and Crystallization Communications·Debanu DasIan A Wilson
Feb 12, 2014·BioMed Research International·Sayangku Nor Ariati Mohamad ArisRaja Noor Zaliha Raja Abd Rahman
Jun 7, 2006·Journal of Molecular Biology·Wataru IwasakiShigeyuki Yokoyama
Apr 8, 2015·Acta Crystallographica. Section F, Structural Biology Communications·Joseph D NgSijay Huang
Nov 26, 2002·Annals of the New York Academy of Sciences·Joseph D Ng
Sep 28, 2017·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Siti Nor Hasmah IshakRaja Noor Zaliha Raja Abd Rahman
Nov 1, 2012·EcoSal Plus·Richard Giegé, Mathias Springer

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