Comparative analysis of steroidal saponins in four Dioscoreae herbs by high performance liquid chromatography coupled with mass spectrometry

Journal of Pharmaceutical and Biomedical Analysis
Long GuoE-Hu Liu

Abstract

Steroidal saponins, which exhibit multiple pharmacological effects, are the major bioactive constituents in herbal medicines from Dioscoreae species. In this study, a sensitive method based on high performance liquid chromatography-mass spectrometry (HPLC-MS) was established and validated for qualitative and quantitative analysis of steroidal saponins in four Dioscoreae herbs including Dioscoreae Nipponica Rhizome (DNR) and Dioscoreae Hypoglaucae Rhizome (DHR), Dioscoreae Spongiosae Rhizome (DSR) and Dioscoreae Rhizome (DR). A total of eleven steroidal saponins were identified by high performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (HPLC-QTOF/MS). Furthermore, seven major steroidal saponins was simultaneous quantified using a high performance liquid chromatography coupled with triple quadrupole mass spectrometry (HPLC-QQQ/MS). The qualitative and quantitative analysis results indicated that the chemical composition of DNR, DHR and DSR samples exhibited a high level of global similarity, while the ingredients in DR varied greatly from the other three herbs. Moreover, principal component analysis (PCA) and hierarchical clustering analysis (HCA) were performed to compare and discriminate ...Continue Reading

References

Feb 6, 2003·The American Journal of Chinese Medicine·Shih-Chang LeeShin Lu
Apr 1, 2006·Journal of Pharmaceutical and Biomedical Analysis·Yang LiuZongwei Cai
Mar 30, 2007·Journal of Chromatography. a·Geoffrey C KiteMonique S J Simmonds
Mar 5, 2013·Carbohydrate Research·Zulfiqar AliIkhlas A Khan

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Citations

Aug 1, 2019·Phytochemical Analysis : PCA·Xuejiao LiWenyuan Gao
Jun 2, 2018·Evidence-based Complementary and Alternative Medicine : ECAM·Longfei YangTonghui Ma
May 1, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Gui-Ya YangLong Guo

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