DOI: 10.1101/514166Jan 7, 2019Paper

Comparative analysis of Synthetic Physical Interactions with the yeast centrosome

BioRxiv : the Preprint Server for Biology
Rowan SM HowellPeter H Thorpe

Abstract

The yeast centrosome or Spindle Pole Body (SPB) is situated in the nuclear membrane, where it nucleates spindle microtubules and acts as a signalling hub. Previously, we used Synthetic Physical Interactions to map the regions of the cell that are sensitive to forced relocalization of proteins across the proteome (Berry et al., 2016). Here, we expand on this work to show that the SPB, in particular, is sensitive to the relocalization of many proteins. This work inspired a new data analysis approach that indicates that relocalization screens may produce more growth defects than previously reported. A set of associations with the SPB result in elevated SPB number and since hyper-proliferation of centrosomes is a hallmark of cancer cells, these associations point the way for the use of yeast models in the study of spindle formation and chromosome segregation in cancer.

Related Concepts

Malignant Neoplasms
Nuclear Envelope
Yeasts
Centrosome
Tumor Cells, Malignant
Cell Proliferation
Spindle Pole Body
Chromosome Segregation
Spindle Cell
Comparative Analysis

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