Comparative analysis of testis transcriptomes from triploid and fertile diploid cyprinid fish

Biology of Reproduction
Kang XuShaojun Liu

Abstract

The fertility of fish is a key factor in fish breeding. RNA-seq is widely used in high-throughput sequencing and provides a rapid method to examine the molecular mechanisms underlying a biological process. To probe fertility-related molecular mechanisms, we obtained testis transcriptomes from diploid and triploid cyprinid fish and tested for differentially expressed genes (DEGs) in the testis. A total of 6730 transcripts were differentially expressed between the triploid and diploid fish. In these transcripts, 2428 transcripts showed reduced expression and 4302 transcripts were overexpressed in triploid fish compared to the diploid fish. Functional analyses revealed that partial genes related to reproductive, developmental, and locomotion processes, and the axoneme, were differentially expressed in triploid fish relative to diploid fish. Pathway analysis indicated that variations in the gene expression levels of the "ubiquinone and other terpenoid-quinone biosynthesis pathway" and the "apoptotic pathway" played a central role in the sterility of triploid male fish. A series of genes (DNAHs, DNAL1, IFTs, and DNAAF1) associated with sperm flagellar assembly and motility, and testis-specific candidate markers (Tcte1, Tekt1, Tekt4,...Continue Reading

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Citations

Aug 9, 2018·Reproduction, Fertility, and Development·Wuhui LiShaojun Liu
Nov 28, 2018·Journal of the Science of Food and Agriculture·Kang XuYulong Yin
Nov 18, 2018·Science China. Life Sciences·Rong ZhouShaojun Liu
Apr 25, 2021·Science China. Life Sciences·Chun ZhangShaojun Liu

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Datasets Mentioned

BETA
SRX699278

Methods Mentioned

BETA
RNA-seq
PCR
transmission electron microscopy

Software Mentioned

DEseq
Inchworm
WEGO
FastQC
R
CD
EST
BLASTx
BLAT
Trinity

Related Concepts

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