Comparative Analysis of the Bufonis Venenum by Using TLC, HPLC, and LC-MS for Different Extraction Methods

Journal of Pharmacopuncture
Hyo-Jae LeeHwa-Seung Yoo

Abstract

Toad venom, called Chan-Su, is a traditional Oriental medicine secreted from the auricular and the skin glands of the Bufo bufo gargarizanz Cantor or B. melanosticus Schneider and has been widely used in China, Korea and other parts of Asia for the treatment of pain, heart conditions, and cancer. We examined the concentrations of the main chemical constituents within a commerciallyavailable toad venom product and compared the levels for different extraction methods. Toad venom was extracted using either cold or hot water, ethanol (EtOH), methanol (MeOH), or ethyl acetate (EtOAc), was fractionated using precipitation or reflux, and was then analyzed using thin layer chromatography (TLC), high-performance liquid chromatography (HTLC), and liquid chroma-tography - mass spectrometry (LC-MS). Individual components were identified by comparisons of the retention times, the ultraviolet spectra, and mass spectras and differences in chemical constituents for different solvents and extraction methods are presented. Components with authentic standards, including serotonin and bufodienolides (cinobufagen, bufalin, cinobufalin, and resibufogenin), were detected. The water extract of toad venom contained the greatest amount of serotonin (75....Continue Reading

References

Sep 11, 1991·Hypertension·M B PamnaniF J Haddy
Dec 1, 1967·Journal of Pharmaceutical Sciences·K K Chen, A Kovaríková
Mar 1, 1994·Chemical & Pharmaceutical Bulletin·T AkizawaV P Butler
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Jul 17, 2010·Journal of Acupuncture and Meridian Studies·Hye Ri YunYung Hyun Choi
Jul 20, 2010·Journal of Ethnopharmacology·Huimin GaoBrigitte Kopp
Sep 28, 2010·Journal of Acupuncture and Meridian Studies·Jung-Sun KimHwa Seung Yoo

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Citations

Mar 22, 2016·Journal of Pharmacopuncture·Kwang-Ho LeeKi-Rok Kwon
Mar 18, 2015·Journal of Pharmacopuncture·Min-Ji ChoiSeung-Hun Cho
Oct 17, 2015·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Daxin ChenJun Peng

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Methods Mentioned

BETA
column chromatography

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