Comparative analysis of the exopolysaccharide biosynthesis gene clusters from four strains of Lactobacillus rhamnosus

Microbiology
B PéantDenis Roy

Abstract

The exopolysaccharide (EPS) biosynthesis gene clusters of four Lactobacillus rhamnosus strains consist of chromosomal DNA regions of 18.5 kb encoding 17 ORFs that are highly similar among the strains. However, under identical conditions, EPS production varies considerably among these strains, from 61 to 1611 mg l(-1). Fifteen genes are co-transcribed starting from the first promoter upstream of wzd. Nevertheless, five transcription start sites were identified by 5'-RACE PCR analysis, and these were associated with promoter sequences upstream of wzd, rmlA, welE, wzr and wzb. Six potential glycosyltransferase genes were identified that account for the assembly of the heptasaccharide repeat unit containing an unusually high proportion of rhamnose. Four genes involved in the biosynthesis of the sugar nucleotide precursor dTDP-L-rhamnose were identified in the EPS biosynthesis locus, which is unusual for lactic acid bacteria. These four genes are expressed from their own promoter (P2), as well as co-transcribed with the upstream EPS genes, resulting in coordinated production of the rhamnose precursor with the enzymes involved in EPS biosynthesis. This is believed to be the first report demonstrating that the sequence, original organ...Continue Reading

References

Aug 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·M J Weickert, G H Chambliss
Aug 1, 1990·FEMS Microbiology Reviews·S Spiro, J R Guest
May 1, 1985·Journal of Dairy Science·N SriranganathanW E Sandine
Jan 1, 1996·Annual Review of Microbiology·I S Roberts
Dec 1, 1996·Trends in Microbiology·P R ReevesP D Rick
Mar 1, 1997·FEMS Microbiology Letters·M J GosalbesG Pérez-Martínez
Sep 1, 1997·Nucleic Acids Research·S F AltschulD J Lipman
Sep 5, 1997·Science·F R BlattnerY Shao
Dec 9, 1997·Applied Microbiology and Biotechnology·J FarrésJ López-Santín
Aug 26, 1998·Proceedings of the National Academy of Sciences of the United States of America·S T EstremR L Gourse
Nov 4, 1998·International Journal of Food Microbiology·D VincentC Déry
Apr 10, 1999·Journal of Bacteriology·F IannelliG Pozzi
May 11, 1999·FEMS Microbiology Reviews·L De Vuyst, B Degeest
Oct 16, 1999·Glycobiology·D Kapitonov, R K Yu
Jun 19, 2002·Glycobiology·Laure JollyFrancesca Stingele
Aug 22, 2002·Archives of Microbiology·Gilbert Thierry LamotheFrancesca Stingele
Oct 9, 2002·Antonie van Leeuwenhoek·Laure JollyJean-Richard Neeser
Feb 5, 2003·Proceedings of the National Academy of Sciences of the United States of America·Michiel KleerebezemRoland J Siezen
Mar 29, 2003·Science·Jian XuJeffrey I Gordon
Jan 1, 1986·Biotechnology Advances·F PaulP Monsan
Dec 19, 2003·Nucleic Acids Research·Alex BatemanSean R Eddy
Jul 28, 1951·Nature·M DUBOISF SMITH
Apr 13, 2004·Nature Biotechnology·John F HeidelbergClaire M Fraser

Citations

Oct 7, 2009·Proceedings of the National Academy of Sciences of the United States of America·Matti KankainenWillem M de Vos
May 1, 2013·Critical Reviews in Food Science and Nutrition·Paulraj KanmaniVenkatesan Arul
Sep 11, 2007·Applied and Environmental Microbiology·Sarah LebeerSigrid C J De Keersmaecker
Sep 2, 2010·Microbiology and Molecular Biology Reviews : MMBR·Ju-Hoon Lee, Daniel J O'Sullivan
Dec 5, 2008·Microbiology and Molecular Biology Reviews : MMBR·Sarah LebeerSigrid C J De Keersmaecker
Jun 6, 2014·PloS One·Maria DimopoulouMarguerite Dols-Lafargue
Apr 18, 2006·Proceedings of the National Academy of Sciences of the United States of America·Marcus J ClaessonPaul W O'Toole
Sep 5, 2014·Microbial Cell Factories·Marie-Pierre Chapot-Chartier, Saulius Kulakauskas
Nov 29, 2011·International Journal of Food Microbiology·Maria DimopoulouMarguerite Dols-Lafargue
Jan 7, 2016·Frontiers in Bioengineering and Biotechnology·Ozlem Ates
Sep 3, 2010·Biotechnology Advances·S BadelP Michaud
Sep 3, 2008·Systematic and Applied Microbiology·Eric HüfnerChristian Hertel
Nov 8, 2015·Archives of Microbiology·Valeria VastanoLidia Muscariello
Sep 4, 2008·Environmental Microbiology·Mya BreitbartDavid Hollander
Sep 25, 2015·Microbial Biotechnology·Enes DertliArjan Narbad
Dec 3, 2014·International Journal of Food Microbiology·Raouf TarebJean-Paul Vernoux
Jun 7, 2014·Frontiers in Microbiology·Marie-Pierre Chapot-Chartier
Jun 9, 2016·International Journal of Molecular Sciences·Huu-Thanh NguyenFrank Delvigne
Apr 16, 2015·Chembiochem : a European Journal of Chemical Biology·Jochen Schmid, Volker Sieber
Dec 8, 2009·Bioscience, Biotechnology, and Biochemistry·Tong DanTadasu Urashima
Aug 24, 2017·FEMS Microbiology Reviews·Ahmad A ZeidanAna Rute Neves

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