Comparative analysis of the exopolysaccharide biosynthesis gene clusters from four strains of Lactobacillus rhamnosus

B PéantDenis Roy


The exopolysaccharide (EPS) biosynthesis gene clusters of four Lactobacillus rhamnosus strains consist of chromosomal DNA regions of 18.5 kb encoding 17 ORFs that are highly similar among the strains. However, under identical conditions, EPS production varies considerably among these strains, from 61 to 1611 mg l(-1). Fifteen genes are co-transcribed starting from the first promoter upstream of wzd. Nevertheless, five transcription start sites were identified by 5'-RACE PCR analysis, and these were associated with promoter sequences upstream of wzd, rmlA, welE, wzr and wzb. Six potential glycosyltransferase genes were identified that account for the assembly of the heptasaccharide repeat unit containing an unusually high proportion of rhamnose. Four genes involved in the biosynthesis of the sugar nucleotide precursor dTDP-L-rhamnose were identified in the EPS biosynthesis locus, which is unusual for lactic acid bacteria. These four genes are expressed from their own promoter (P2), as well as co-transcribed with the upstream EPS genes, resulting in coordinated production of the rhamnose precursor with the enzymes involved in EPS biosynthesis. This is believed to be the first report demonstrating that the sequence, original organ...Continue Reading


Aug 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·M J Weickert, G H Chambliss
Aug 1, 1990·FEMS Microbiology Reviews·S Spiro, J R Guest
May 1, 1985·Journal of Dairy Science·N SriranganathanW E Sandine
Jan 1, 1996·Annual Review of Microbiology·I S Roberts
Dec 1, 1996·Trends in Microbiology·P R ReevesP D Rick
Mar 1, 1997·FEMS Microbiology Letters·M J GosalbesG Pérez-Martínez
Sep 1, 1997·Nucleic Acids Research·S F AltschulD J Lipman
Sep 5, 1997·Science·F R BlattnerY Shao
Dec 9, 1997·Applied Microbiology and Biotechnology·J FarrésJ López-Santín
Aug 26, 1998·Proceedings of the National Academy of Sciences of the United States of America·S T EstremR L Gourse
Nov 4, 1998·International Journal of Food Microbiology·D VincentC Déry
Apr 10, 1999·Journal of Bacteriology·F IannelliG Pozzi
May 11, 1999·FEMS Microbiology Reviews·L De Vuyst, B Degeest
Oct 16, 1999·Glycobiology·D Kapitonov, R K Yu
Jun 19, 2002·Glycobiology·Laure JollyFrancesca Stingele
Aug 22, 2002·Archives of Microbiology·Gilbert Thierry LamotheFrancesca Stingele
Oct 9, 2002·Antonie van Leeuwenhoek·Laure JollyJean-Richard Neeser
Feb 5, 2003·Proceedings of the National Academy of Sciences of the United States of America·Michiel KleerebezemRoland J Siezen
Mar 29, 2003·Science·Jian XuJeffrey I Gordon
Jan 1, 1986·Biotechnology Advances·F PaulP Monsan
Dec 19, 2003·Nucleic Acids Research·Alex BatemanSean R Eddy
Jul 28, 1951·Nature·M DUBOISF SMITH
Apr 13, 2004·Nature Biotechnology·John F HeidelbergClaire M Fraser


Oct 7, 2009·Proceedings of the National Academy of Sciences of the United States of America·Matti KankainenWillem M de Vos
May 1, 2013·Critical Reviews in Food Science and Nutrition·Paulraj KanmaniVenkatesan Arul
Sep 11, 2007·Applied and Environmental Microbiology·Sarah LebeerSigrid C J De Keersmaecker
Sep 2, 2010·Microbiology and Molecular Biology Reviews : MMBR·Ju-Hoon Lee, Daniel J O'Sullivan
Dec 5, 2008·Microbiology and Molecular Biology Reviews : MMBR·Sarah LebeerSigrid C J De Keersmaecker
Jun 6, 2014·PloS One·Maria DimopoulouMarguerite Dols-Lafargue
Apr 18, 2006·Proceedings of the National Academy of Sciences of the United States of America·Marcus J ClaessonPaul W O'Toole
Sep 5, 2014·Microbial Cell Factories·Marie-Pierre Chapot-Chartier, Saulius Kulakauskas
Nov 29, 2011·International Journal of Food Microbiology·Maria DimopoulouMarguerite Dols-Lafargue
Jan 7, 2016·Frontiers in Bioengineering and Biotechnology·Ozlem Ates
Sep 3, 2010·Biotechnology Advances·S BadelP Michaud
Sep 3, 2008·Systematic and Applied Microbiology·Eric HüfnerChristian Hertel
Nov 8, 2015·Archives of Microbiology·Valeria VastanoLidia Muscariello
Sep 4, 2008·Environmental Microbiology·Mya BreitbartDavid Hollander
Sep 25, 2015·Microbial Biotechnology·Enes DertliArjan Narbad
Dec 3, 2014·International Journal of Food Microbiology·Raouf TarebJean-Paul Vernoux
Jun 7, 2014·Frontiers in Microbiology·Marie-Pierre Chapot-Chartier
Jun 9, 2016·International Journal of Molecular Sciences·Huu-Thanh NguyenFrank Delvigne
Apr 16, 2015·Chembiochem : a European Journal of Chemical Biology·Jochen Schmid, Volker Sieber
Dec 8, 2009·Bioscience, Biotechnology, and Biochemistry·Tong DanTadasu Urashima
Aug 24, 2017·FEMS Microbiology Reviews·Ahmad A ZeidanAna Rute Neves

Related Concepts

Trending Feeds


Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Hereditary Sensory Autonomic Neuropathy

Hereditary Sensory Autonomic Neuropathies are a group of inherited neurodegenerative disorders characterized clinically by loss of sensation and autonomic dysfunction. Here is the latest research on these neuropathies.

Glut1 Deficiency

Glut1 deficiency, an autosomal dominant, genetic metabolic disorder associated with a deficiency of GLUT1, the protein that transports glucose across the blood brain barrier, is characterized by mental and motor developmental delays and infantile seizures. Follow the latest research on Glut1 deficiency with this feed.

Regulation of Vocal-Motor Plasticity

Dopaminergic projections to the basal ganglia and nucleus accumbens shape the learning and plasticity of motivated behaviors across species including the regulation of vocal-motor plasticity and performance in songbirds. Discover the latest research on the regulation of vocal-motor plasticity here.

Neural Activity: Imaging

Imaging of neural activity in vivo has developed rapidly recently with the advancement of fluorescence microscopy, including new applications using miniaturized microscopes (miniscopes). This feed follows the progress in this growing field.

Nodding Syndrome

Nodding Syndrome is a neurological and epileptiform disorder characterized by psychomotor, mental, and growth retardation. Discover the latest research on Nodding Syndrome here.

LRRK2 & Microtubules

Mutations in the LRRK2 gene are risk-factors for developing Parkinson’s disease (PD). LRRK2 mutations in PD have been shown to enhance its association with microtubules. Here is the latest research.