Comparative analysis of the global transcriptome of Anopheles funestus from Mali, West Africa.

PloS One
Andrew C SerazinNora J Besansky

Abstract

Anopheles funestus is a principal vector of malaria across much of tropical Africa and is considered one of the most efficient of its kind, yet studies of this species have lagged behind those of its broadly sympatric congener, An. gambiae. In aid of future genomic sequencing of An. funestus, we explored the whole body transcriptome, derived from mixed stage progeny of wild-caught females from Mali, West Africa. Here we report the functional annotation and comparative genomics of 2,005 expressed sequence tags (ESTs) from An. funestus, which were assembled with a previous EST set from adult female salivary glands from the same mosquito. The assembled ESTs provided for a nonredundant catalog of 1,035 transcripts excluding mitochondrial sequences. Comparison of the An. funestus and An. gambiae transcriptomes using computational and macroarray approaches revealed a high degree of sequence identity despite an estimated 20-80 MY divergence time between lineages. A phylogenetically broader comparative genomic analysis indicated that the most rapidly evolving proteins--those involved in immunity, hematophagy, formation of extracellular structures, and hypothetical conserved proteins--are those that probably play important roles in how ...Continue Reading

References

Sep 1, 1997·Nucleic Acids Research·S F AltschulD J Lipman
Dec 11, 1999·Nucleic Acids Research·J SchultzP Bork
Dec 11, 1999·Nucleic Acids Research·A BatemanE L Sonnhammer
Mar 15, 2001·Journal of Neuroscience Methods·L W Whitney, K G Becker
Dec 26, 2001·Nucleic Acids Research·Aron Marchler-BauerStephen H Bryant
Aug 27, 2002·Annual Review of Entomology·Jose M C Ribeiro, Ivo M B Francischetti
Oct 5, 2002·Science·Hilary RansonRené Feyereisen
Oct 5, 2002·Science·Igor V SharakhovNora J Besansky
Jun 27, 2003·Insect Biochemistry and Molecular Biology·Jesus G ValenzuelaJosé M C Ribeiro
Sep 13, 2003·BMC Bioinformatics·Roman L TatusovDarren A Natale
Dec 12, 2003·Bioinformatics·Julio RozasRicardo Rozas
Jul 10, 2004·Insect Biochemistry and Molecular Biology·M Coetzee, D Fontenille
Oct 14, 2004·Nucleic Acids Research·Michael Y Galperin, Eugene V Koonin
Jan 7, 2005·BMC Bioinformatics·David A RaskoJacques Ravel
Aug 24, 2006·Annual Review of Entomology·Xianchun LiMay R Berenbaum
Jan 25, 2007·Insect Biochemistry and Molecular Biology·Eric CalvoJosé M C Ribeiro
Nov 8, 2007·Nature Genetics·Timothy B SacktonAndrew G Clark
Nov 19, 2008·Nature Reviews. Genetics·Zhong WangMichael Snyder

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Citations

May 21, 2014·Comparative Biochemistry and Physiology. Part D, Genomics & Proteomics·Nguyen Minh ThanhPeter Mather
Jan 16, 2013·Annual Review of Entomology·Maureen Coetzee, Lizette L Koekemoer
Nov 3, 2010·The American Journal of Tropical Medicine and Hygiene·Maria V SharakhovaIgor V Sharakhov
Nov 15, 2012·BMC Genomics·Albin FontaineLionel Almeras
Jul 27, 2011·Journal of Insect Physiology·Rajnikant DixitArun Sharma

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Methods Mentioned

BETA
PCR
electrophoresis
environmental stresses

Software Mentioned

Genespring
BLASTN
BLASTP
Assembler
JMCR
BLAST
TBLASTX
DNAStar SeqMan II
Excel
Jaguar

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