Comparative Analysis of the Liver and Spleen Transcriptomes between Holstein and Yunnan Humped Cattle

Animals : an Open Access Journal From MDPI
Yanyan ChenShanyuan Chen

Abstract

Previous studies have shown that Yunnan humped cattle have higher disease resistance than pure taurine cattle, such as Holsteins. However, there exists limited information about the molecular genetic basis underlying disease resistance differences between them. The objective of this study was to compare differentially expressed genes (DEGs) in the liver and spleen tissues of Holstein and Yunnan humped cattle through comparative transcriptome analysis, using RNA-sequencing. In total, 1564 (647 up- and 917 down-regulated genes) and 1530 (716 up- and 814 down-regulated genes) DEGs were obtained in the liver and spleen tissues of Holstein and Yunnan humped cattle comparison groups, respectively. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis showed that the DEGs were mainly associated with the RIG-I signaling pathway, immune responses, major histocompatibility complex (MHC) class I protein complex and complement activation, human T-cell lymphotropic virus type-I (HTLV-I) infection. Some genes related to immune function, such as C1QB, CD55, MASP2, C4BPA, MAVS, NOD2, and CD46, were up-regulated in Yunnan humped cattle, while C2, SERPING1, SERPINE1, TIRAP, TLR2, and TLR6 were down-regulated. ...Continue Reading

References

Mar 29, 1994·Proceedings of the National Academy of Sciences of the United States of America·R T LoftusP Cunningham
Feb 24, 2001·Journal of Gastroenterology and Hepatology·M NakagawaT Tsuji
Apr 27, 2001·Nature·C S TroyD G Bradley
Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Nov 25, 2003·Nature Reviews. Genetics·Michael W BrufordGordon Luikart
Apr 23, 2004·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Zahra MadjdSarah E Pinder
Jun 3, 2008·Nature Methods·Ali MortazaviBarbara Wold
Sep 2, 2008·Blood·Bruce A Beutler
Sep 2, 2008·Comparative Immunology, Microbiology and Infectious Diseases·Elizabeth A CatesDouglas D Bannerman
Oct 8, 2008·BMC Genetics·Abigail R FreemanDaniel G Bradley
Nov 19, 2008·Nature Reviews. Genetics·Zhong WangMichael Snyder
Dec 19, 2009·Proceedings of the National Academy of Sciences of the United States of America·Christopher M SeaburyJames E Womack
Apr 2, 2010·Molecular Ecology·Diethard TautzDetlef Weigel
Feb 7, 2012·Comparative Immunology, Microbiology and Infectious Diseases·Martin VordermeierDouglas B Young
Feb 14, 2012·European Journal of Immunology·Yetao WangXin Ye
Sep 14, 2013·Frontiers in Immunology·Ranjeet Singh MahlaHimanshu Kumar
Apr 4, 2014·Bioinformatics·Anthony M BolgerBjoern Usadel
Jan 28, 2015·Chemical Research in Toxicology·Dingfeng WuRuixin Zhu
Jun 23, 2015·Clinical and Experimental Immunology·Y M MosaadI M Fawzy
Jul 4, 2017·Biochemical and Biophysical Research Communications·Xi WangWenzhong Liu
Apr 19, 2018·Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology·Rui PengFangdong Zou
May 18, 2018·Current Opinion in Immunology·Masatake Tanaka, Yasuko Iwakiri
Oct 24, 2018·Nature Communications·Sonya A MacParlandIan D McGilvray
Feb 19, 2019·Xenotransplantation·Dengke PanShaoping Deng

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Methods Mentioned

BETA
Illumina sequencing
RNA-seq
PCR

Software Mentioned

DAVID
Trimmomatic
TopHat2
DESeq

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