PMID: 9524066Apr 2, 1998Paper

Comparative analysis of the sequences and three-dimensional models of human procarboxypeptidases A1, A2 and B

Biological Chemistry
P AloyF X Avilés

Abstract

A full-length cDNA clone for preprocarboxypeptidase B from human pancreas has been isolated and sequenced. The open reading frame is 1254 bp in length, encoding a protein of 417 amino acids that includes a leader signal peptide of 15 amino acids and a 95-amino acid-long pro-segment. It contains two differences when compared to the sequence reported for pancreas-specific protein, a human serum marker for acute pancreatitis identified as procarboxypeptidase B. The main difference is a previously unreported Cys at position 138, which is needed for the formation of one of the three disulphide bridges. Sequence alignments between human procarboxypeptidases A1, A2 and B and other known forms show that the most conserved region is the enzyme moiety followed by the globular domain of the pro-segment. The maximum variability is found in the connecting region between moieties. The known three-dimensional structures of procarboxypeptidases from bovine and porcine species have been used to model all three human procarboxypeptidases and also to estimatethe interaction energies between the different parts of the molecules, in an attempt to gain insight into the structural features responsible for the differences observed in the functionality...Continue Reading

References

Dec 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·F SangerA R Coulson
Oct 1, 1992·The Biochemical Journal·L CatasúsA Puigserver
Apr 30, 1991·Biochemical and Biophysical Research Communications·B OlivaO Tapia
Jan 1, 1987·Methods in Enzymology·R C MierendorfR A Young
Feb 15, 1989·European Journal of Biochemistry·R PascualF X Aviles
Dec 5, 1993·Journal of Molecular Biology·A Sali, T L Blundell

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Citations

Sep 21, 2000·European Journal of Biochemistry·A M FernándezP L Mateo
Oct 25, 2000·Protein Science : a Publication of the Protein Society·V VillegasL Serrano
Sep 10, 2015·Journal of Medical Entomology·Watcharakorn MongkolAnchanee Kubera
Apr 11, 2018·Proceedings of the National Academy of Sciences of the United States of America·Maria C Garcia-GuerreroDavid Reverter

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