Comparative analysis of trehalose production by Debaryomyces hansenii and Saccharomyces cerevisiae under saline stress

Extremophiles : Life Under Extreme Conditions
Juan Carlos González-HernándezA Peña

Abstract

The comparative analysis of growth, intracellular content of Na+ and K+, and the production of trehalose in the halophilic Debaryomyces hansenii and Saccharomyces cerevisiae were determined under saline stress. The yeast species were studied based on their ability to grow in the absence or presence of 0.6 or 1.0 M NaCl and KCl. D. hansenii strains grew better and accumulated more Na+ than S. cerevisiae under saline stress (0.6 and 1.0 M of NaCl), compared to S. cerevisiae strains under similar conditions. By two methods, we found that D. hansenii showed a higher production of trehalose, compared to S. cerevisiae; S. cerevisiae active dry yeast contained more trehalose than a regular commercial strain (S. cerevisiae La Azteca) under all conditions, except when the cells were grown in the presence of 1.0 M NaCl. In our experiments, it was found that D. hansenii accumulates more glycerol than trehalose under saline stress (2.0 and 3.0 M salts). However, under moderate NaCl stress, the cells accumulated more trehalose than glycerol. We suggest that the elevated production of trehalose in D. hansenii plays a role as reserve carbohydrate, as reported for other microorganisms.

References

Jan 1, 1978·Advances in Microbial Physiology·A D Brown
Jan 1, 1978·CRC Critical Reviews in Microbiology·S T Bayley, R A Morton
Jan 1, 1992·Advances in Microbial Physiology·A Blomberg, L Adler
Oct 1, 1990·Antonie van Leeuwenhoek·A Wiemken
Jan 1, 1974·Advances in Carbohydrate Chemistry and Biochemistry·A D Elbein
Oct 1, 1972·Journal of General Microbiology·A D Brown, J R Simpson
Dec 7, 1982·Biochemistry·T Arakawa, S N Timasheff
Jan 1, 1995·Trends in Biochemical Sciences·J M Thevelein, S Hohmann
Jul 1, 1993·Analytical Biochemistry·B U StambukP S de Araujo
Jan 1, 1996·International Review of Cytology·R Serrano
Oct 6, 1997·FEMS Microbiology Letters·J A JorgeH F Terenzi
Nov 5, 1997·Applied and Environmental Microbiology·C PristaJ Ramos
Nov 18, 2000·Archives of Microbiology·J C Argüelles
Dec 1, 2000·Applied and Environmental Microbiology·H IwahashiK Obuchi
Jan 22, 2002·Metabolic Engineering·Denis RonteinAndrew D Hanson
Apr 30, 2004·Yeast·J C González-HernándezA Peña
Jun 8, 1984·Science·D Le RudulierR C Valentine

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Citations

Apr 16, 2005·Current Opinion in Biotechnology·Gertien J Smits, Stanley Brul
May 23, 2012·Toxicological Sciences : an Official Journal of the Society of Toxicology·Lan GuoPaul Russell
Mar 7, 2008·Current Microbiology·Ravi K AsthanaSureshwar P Singh
Sep 15, 2009·Mycological Research·Nina Gunde-CimermanAna Plemenitas
Aug 26, 2009·FEMS Yeast Research·Martha CalahorraAntonio Peña
Aug 30, 2008·FEMS Yeast Research·Norma Silvia SánchezAntonio Peña
Jan 7, 2017·PloS One·Wilson Garcia-NetoLuis Alberto Luévano-Martínez
May 10, 2007·Current Microbiology·Sathyanarayana N GummadiC N A Aneesh
Apr 9, 2008·AIDS and Behavior·Luiz Oscar Cardoso FerreiraWilli McFarland
Jun 3, 2021·Journal of Fungi·Eya Caridad Rodríguez-PupoMaría Del Rayo Sánchez-Carbente

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