Comparative analysis of two C-terminal kinesin motor proteins: KIFC1 and KIFC5A

Cell Motility and the Cytoskeleton
Yuguo Zhang, Ann O Sperry

Abstract

We have taken advantage of the close structural relationship between two C-terminal motors, KIFC5A and KIFC1, to examine the sequence requirements for targeting of these two motors within the cell. Although KIFC5A and KIFC1 are almost identical in their motor and stalk domains, they differ in well-defined regions of their tail domains. Specific antisera to these motors were used to determine their localization to distinct subcellular compartments, the spindle for KIFC5A or membranous organelles for KIFC1. In addition to defining the intracellular localization of KIFC1, the reactivity of the KIFC1 antibody demonstrates that this motor contains a frame shift with respect to KIFC5A and is likely the product of a separate gene. The divergent tail domains of these motors are predicted to harbor specific information that directs them to their correct intracellular targets. In order to define the sequences responsible for the differential localization of these two motors, GFP was fused to motors with various tail deletions and their localization visualized after transfection. We were able to identify distinct sequences in each motor responsible for its unique cellular localization. The KIFC5A tail contains a 43 amino acid sequence wit...Continue Reading

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Citations

Mar 16, 2007·Molecular Biology of the Cell·Sangeeta NathAllan W Wolkoff
Jun 18, 2010·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·Da-Hui Wang, Wan-Xi Yang
Mar 23, 2010·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Wei WangWan-Xi Yang
Jun 2, 2007·Toxicology and Applied Pharmacology·Dale W SicklesMarvin Friedman
Sep 24, 2010·Cytoskeleton·M A SilvermanG Banker
Jul 3, 2017·Journal of Cell Science·Zhen-Yu She, Wan-Xi Yang
Apr 23, 2016·Oncotarget·Yu-Xi Xiao, Wan-Xi Yang
May 28, 2019·Cell Death & Disease·Ya-Lan Wei, Wan-Xi Yang
May 25, 2021·Biochemistry and Biophysics Reports·Vincent Cutillas, Christopher A Johnston

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