Comparative Analysis of Virulence and Toxin Expression of Vancomycin-Intermediate and Vancomycin-Sensitive Staphylococcus aureus Strains

Frontiers in Microbiology
Ye JinYonghong Xiao

Abstract

Previous studies on vancomycin-intermediate Staphylococcus aureus (VISA) have mainly focused on drug resistance, the evolution of differences in virulence between VISA and vancomycin-sensitive S. aureus (VSSA) requires further investigation. To address this issue, in this study, we compared the virulence and toxin profiles of pair groups of VISA and VSSA strains, including a series of vancomycin-resistant induced S. aureus strains-SA0534, SA0534-V8, and SA0534-V16. We established a mouse skin infection model to evaluate the invasive capacity of VISA strains, and found that although mice infected with VISA had smaller-sized abscesses than those infected with VSSA, the abscesses persisted for a longer period (up to 9 days). Infection with VISA strains was associated with a lower mortality rate in Galleria mellonella larvae compared to infection with VSSA strains (≥ 40% vs. ≤ 3% survival at 28 h). Additionally, VISA were more effective in colonizing the nasal passage of mice than VSSA, and in vitro experiments showed that while VISA strains were less virulent they showed enhanced intracellular survival compared to VSSA strains. RNA sequencing of VISA strains revealed significant differences in the expression levels of the agr, hla...Continue Reading

References

Jul 21, 1988·The New England Journal of Medicine·R LeclercqP Courvalin
Jul 1, 1972·The Journal of Experimental Medicine·J HawigerR M Des Prez
May 8, 1999·Science·C Walsh
Aug 16, 2001·Drug Resistance Updates : Reviews and Commentaries in Antimicrobial and Anticancer Chemotherapy·Michael R. Yeaman, Arnold S. Bayer
Oct 27, 2001·The Journal of Antimicrobial Chemotherapy·S Boyle-VavraR S Daum
Jan 18, 2002·Infection and Immunity·Thanh LuongChia Y Lee
Apr 12, 2002·The Lancet Infectious Diseases·Duarte C OliveiraHermínia de Lencastre
May 2, 2002·Proceedings of the National Academy of Sciences of the United States of America·Scott D KobayashiFrank R DeLeo
Jan 9, 2003·Journal of Clinical Microbiology·Longzhu CuiKeiichi Hiramatsu
Feb 16, 2005·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Stan Deresinski
Sep 9, 2005·The Journal of Immunology : Official Journal of the American Association of Immunologists·Jovanka M VoyichFrank R DeLeo
Dec 3, 2005·Nature Reviews. Microbiology·Timothy J Foster
Jan 8, 2009·The Journal of Infectious Diseases·Anton Y PelegGeorge M Eliopoulos
May 20, 2009·Antimicrobial Agents and Chemotherapy·Yuki KatayamaKeiichi Hiramatsu
Aug 19, 2011·The Journal of Infectious Diseases·Scott D KobayashiFrank R Deleo
Sep 17, 2011·Virulence·Nathan K ArcherMark E Shirtliff
Aug 4, 2012·The Journal of Infectious Diseases·Davida S SmythBo Shopsin
Dec 29, 2012·Journal of Visualized Experiments : JoVE·Nalini RamaraoDidier Lereclus
Aug 15, 2014·Molecular Microbiology·E Magda BarbuMagnus Höök

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Methods Mentioned

BETA
Enzyme-Linked Immunosorbent Assay
ELISA
Assay
PCR
RNA-seq

Software Mentioned

Prism
GraphPad

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