Comparative analysis of virus-derived small RNAs within cassava (Manihot esculenta Crantz) infected with cassava brown streak viruses

Virus Research
Emmanuel OgwokNigel J Taylor

Abstract

Infection of plant cells by viral pathogens triggers RNA silencing, an innate antiviral defense mechanism. In response to infection, small RNAs (sRNAs) are produced that associate with Argonaute (AGO)-containing silencing complexes which act to inactivate viral genomes by posttranscriptional gene silencing (PTGS). Deep sequencing was used to compare virus-derived small RNAs (vsRNAs) in cassava genotypes NASE 3, TME 204 and 60444 infected with the positive sense single-stranded RNA (+ssRNA) viruses cassava brown streak virus (CBSV) and Ugandan cassava brown streak virus (UCBSV), the causal agents of cassava brown streak disease (CBSD). An abundance of 21-24nt vsRNAs was detected and mapped, covering the entire CBSV and UCBSV genomes. The 21nt vsRNAs were most predominant, followed by the 22 nt class with a slight bias toward sense compared to antisense polarity, and a bias for adenine and uracil bases present at the 5'-terminus. Distribution and frequency of vsRNAs differed between cassava genotypes and viral genomes. In susceptible genotypes TME 204 and 60444, CBSV-derived sRNAs were seen in greater abundance than UCBSV-derived sRNAs. NASE 3, known to be resistant to UCBSV, accumulated negligible UCBSV-derived sRNAs but high po...Continue Reading

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Citations

May 28, 2019·Frontiers in Plant Science·Samar SheatStephan Winter
Mar 30, 2017·Archives of Virology·Yanhong QiuShuifang Zhu
Jun 25, 2017·Annual Review of Virology·Chrissie Rey, Hervé Vanderschuren
Sep 10, 2017·Molecular Plant Pathology·Katie R TomlinsonGary D Foster
Jun 14, 2020·Scientific Reports·Deepti NigamHernan Garcia-Ruiz

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Methods Mentioned

BETA
PCR
electrophoresis

Software Mentioned

BEDTools
Cutadapt
QIIME
Bowtie

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