Comparative and network-based proteomic analysis of low dose ethanol- and lipopolysaccharide-induced macrophages

PloS One
Abu Hena M KamalSaiful M Chowdhury

Abstract

Macrophages are specialized phagocytes that play an essential role in inflammation, immunity, and tissue repair. Profiling the global proteomic response of macrophages to microbial molecules such as bacterial lipopolysaccharide is key to understanding fundamental mechanisms of inflammatory disease. Ethanol is a widely abused substance that has complex effects on inflammation. Reports have indicated that ethanol can activate or inhibit the lipopolysaccharide receptor, Toll-like Receptor 4, in different settings, with important consequences for liver and neurologic inflammation, but the underlying mechanisms are poorly understood. To profile the sequential effect of low dose ethanol and lipopolysaccharide on macrophages, a gel-free proteomic technique was applied to RAW 264.7 macrophages. Five hundred four differentially expressed proteins were identified and quantified with high confidence using ≥ 5 peptide spectral matches. Among these, 319 proteins were shared across all treatment conditions, and 69 proteins were exclusively identified in ethanol-treated or lipopolysaccharide-stimulated cells. The interactive impact of ethanol and lipopolysaccharide on the macrophage proteome was evaluated using bioinformatics tools, enabling ...Continue Reading

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Citations

Sep 27, 2019·Journal of Leukocyte Biology·Arjun SukumaranJennifer Geddes-McAlister
May 1, 2019·International Journal of Molecular Sciences·Yashwanth SubbannayyaRichard K Kandasamy
Feb 8, 2020·Journal of the American Society for Mass Spectrometry·Jayanta K ChakrabartySaiful M Chowdhury

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Datasets Mentioned

BETA
PXD006990

Methods Mentioned

BETA
protein assay
reversed-phase chromatography
PCR
ubiquitination
proteomic profiling

Software Mentioned

MeV
Proteome Discoverer
Xcalibur
PANTHER
GraphPad
Ingenuity Knowledge Base
SEQUEST
GraphPad Prism
IPA

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