Comparative antibiotic eradication of mycoplasma infections from continuous cell lines

In Vitro Cellular & Developmental Biology. Animal
Cord C Uphoff, Hans G Drexler

Abstract

Accumulating data implicate mycoplasma contamination as the single biggest problem in the culture of continuous cell lines. Mycoplasma infection can affect virtually every parameter and functional activity of the eukaryotic cells. A successful alternative to discarding infected cultures is to attempt to eliminate the contaminants by treatment with specific and efficient antimycoplasma antibiotics. The addition of antibiotics to the culture medium during a limited period of time (1-3 wk) is a simple, inexpensive, and very practical approach for decontaminating continuous cell lines. Here, we examined the effectiveness of several antibiotic treatment protocols that we have employed routinely in our cell lines bank. On an aggregate, 673 cultures from 236 chronically mycoplasma-positive cell lines were exposed to one of the following five antibiotic regimens: mycoplasma removal agent (quinolone; a 1-wk treatment), enrofloxacin (quinolone; 1 wk), sparfloxacin (quinolone; 1 wk), ciprofloxacin (quinolone; 2 wk), and BM-Cyclin (alternating tiamulin and minocycline; 3 wk). The mycoplasma infection was permanently (as determined by three solid mycoplasma detection assays) eliminated by the various antibiotics in 66-85% of the cultures tr...Continue Reading

References

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Jun 8, 1989·Nature·R J HayT R Chen
May 1, 1994·In Vitro Cellular & Developmental Biology. Animal·H G DrexlerC C Uphoff
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Citations

Mar 19, 2014·Doklady. Biochemistry and Biophysics·E S MedvedevaV M Chernov
Aug 20, 2008·BMC Research Notes·Haitian ZhaoPhilippe Marambaud
Oct 24, 2012·Journal of Biomedicine & Biotechnology·Cord C UphoffHans G Drexler
Feb 13, 2010·Biologicals : Journal of the International Association of Biological Standardization·Martha FolmsbeeMorven McAlister
Jul 25, 2006·Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas·J TimenetskyE Mettifogo
Nov 20, 2008·Current Protocols in Microbiology·Nicole C AmmermanAbdu F Azad
Apr 16, 2014·Current Protocols in Molecular Biology·Cord C Uphoff, Hans G Drexler
Aug 2, 2019·Cancer Cell International·Fei ZhouXiaoming Yang
Jul 27, 2018·Fish Physiology and Biochemistry·Raj KumarThangaraj Raja Swaminathan
Jul 28, 2019·Metallomics : Integrated Biometal Science·Lukáš MoráňPetr Vaňhara

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Methods Mentioned

BETA
PCR

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