PMID: 3529606Jul 1, 1986

Comparative antigenic analysis of extracellular proteins of Bacteroides nodosus isolated from virulent and benign ovine footrot

Veterinary Microbiology
W K Yong, L M Gordon

Abstract

Antigens in the extracellular protein (ECP) complexes of Bacteroides nodosus, isolated from sheep with either benign or virulent footrot, were studied by immunoelectrophoresis (IEP). Rabbit antisera against ECP from virulent and benign strains, were used in homologous and heterologous crossed IEP. Four precipitin peaks unique to the virulent strain, and five peaks unique to the benign strain were identified. In an attempt to characterize the different antigens in ECP, rabbit antisera were raised against an outer membrane protein (OMP, mol. wt. 35 000 daltons), pili and various proteases of virulent and benign strains of B. nodosus. No precipitin band was observed when ECP from both B. nodosus strains were reacted against anti-OMP and anti-pilus antisera. However, single precipitin bands unique to one protease from the benign strain and one protease from the virulent strain were identified. The results suggest that specific antigens other than proteases or pili are important in determining whether a B. nodosus isolate is virulent or benign.

References

Jan 1, 1979·Australian Veterinary Journal·L J Depiazzi, R B Richards
Jan 1, 1973·Journal of Comparative Pathology·J R Egerton
Aug 1, 1969·Scandinavian Journal of Clinical and Laboratory Investigation·J Kröll
Jan 1, 1983·Journal of General Microbiology·D Every, T M Skerman

Related Concepts

Antigens, Bacterial
Bacterial Proteins
Bacteroides
Bacteroides Infections
SDS-PAGE
Foot Rot
Immunoelectrophoresis
Peptide Hydrolases
Dall Sheep
Sheep Diseases

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