Comparative approach to the evaluation of hemoglobin A2 by two different methods: high-performance liquid chromatography and DE-52 microchromatography

Acta Haematologica
P SamperiG Schilirò

Abstract

Hb A2 was determined in 477 subjects: 77 were affected by iron deficiency anemia, 172 were carriers of beta-thalassemia trait and 228 were normal controls. Hb A2 was determined by both DE-52 microchromatography and high-performance liquid chromatography (HPLC). The analysis of the data by linear regression demonstrated that the methods furnish overlapping results. Our findings show that HPLC is a rapid and easily reproduced method which allows a quantitative and qualitative discrimination of the various Hb fractions, making it a valid tool in screening programs for hemoglobinopathies.

Citations

Apr 17, 1998·International Journal of Gynaecology and Obstetrics : the Official Organ of the International Federation of Gynaecology and Obstetrics·C WanapirakA Tuggapichitti
Jan 1, 1991·Hemoglobin·G SchilliròR Testa
Apr 17, 1999·Scandinavian Journal of Clinical and Laboratory Investigation·E J Harthoorn-LasthuizenM M Langenhuijsen
Mar 25, 2000·Electrophoresis·Z K ShihabiH K Daugherty
Apr 5, 2013·Prenatal Diagnosis·T TongsongSavitree Pranpanus

Related Concepts

Asiderotic Anemia
Chromatography, DEAE-Cellulose
High Pressure Liquid Chromatography Procedure
Hemoglobin A
Hemoglobin A2
Heterozygote Detection
Thalassemia

Related Feeds

Anemia

Anemia develops when your blood lacks enough healthy red blood cells. Anemia of inflammation (AI, also called anemia of chronic disease) is a common, typically normocytic, normochromic anemia that is caused by an underlying inflammatory disease. Here is the latest research on anemia.

Related Papers

International Journal of Gynaecology and Obstetrics : the Official Organ of the International Federation of Gynaecology and Obstetrics
C WanapirakT Tongsong
The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society
M L OsterhoutK Horiuchi
© 2021 Meta ULC. All rights reserved